3IZD

Model of the large subunit RNA expansion segment ES27L-out based on a 6.1 A cryo-EM map of Saccharomyces cerevisiae translating 80S ribosome. 3IZD is a small part (an expansion segment) which is in an alternative conformation to what is in already 3IZF.

Summary for 3IZD

• Entry DOI: 10.2210/pdb3izd/pdb
• Related: 3IZ5 3IZ6 3IZ7 3IZ9
• EMDB information: 1667 1811
• Descriptor: rRNA expansion segment ES27L in an "out" conformation (1 entity in total)
• Functional Keywords: eukaryotic ribosome, homology modelling, de novo modeling, ribosomal rna, rrna, rna expansion segments, ribosome
• Biological source: Saccharomyces cerevisiae (yeast)
• Total number of polymer chains: 1
• Total formula weight: 48721.58

Authors

Armache, J.-P.,Jarasch, A.,Anger, A.M.,Villa, E.,Becker, T.,Bhushan, S.,Jossinet, F.,Habeck, M.,Dindar, G.,Franckenberg, S.,Marquez, V.,Mielke, T.,Thomm, M.,Berninghausen, O.,Beatrix, B.,Soeding, J.,Westhof, E.,Wilson, D.N.,Beckmann, R. (deposition date: 2010-10-13, release date: 2010-12-01, Last modification date: 2024-02-21)

Primary citation

Armache, J.P.,Jarasch, A.,Anger, A.M.,Villa, E.,Becker, T.,Bhushan, S.,Jossinet, F.,Habeck, M.,Dindar, G.,Franckenberg, S.,Marquez, V.,Mielke, T.,Thomm, M.,Berninghausen, O.,Beatrix, B.,Soding, J.,Westhof, E.,Wilson, D.N.,Beckmann, R.
Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.
Proc.Natl.Acad.Sci.USA, 107:19748-19753, 2010

PubMed Abstract: Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.

PubMed: 20980660
DOI: 10.1073/pnas.1009999107

Experimental method

ELECTRON MICROSCOPY (8.6 Å)