3IY8

Leishmania tarentolae Mitonchondrial Ribosome small subunit

3IY8 の概要

• エントリーDOI: 10.2210/pdb3iy8/pdb
• EMDBエントリー: 5113
• 分子名称: Leishmania tarentolae mitochondrial small subunit, 30S ribosomal protein S17, 30S ribosomal protein S18, ... (11 entities in total)
• 機能のキーワード: leishmania tarentolae, mitochondrial ribosome, cryoem, minimal rna., antibiotic resistance, ribonucleoprotein, ribosomal protein, rna-binding, rrna-binding, repressor, translation regulation, trna-binding, methylation, endonuclease, hydrolase, nuclease, ribosome
• 由来する生物種: Leishmania tarentolae; 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 289516.58

構造登録者

Sharma, M.R.,Booth, T.M.,Simpson, L.,Maslov, D.A.,Agrawal, R.K. (登録日: 2009-04-16, 公開日: 2009-07-07, 最終更新日: 2024-02-21)

主引用文献

Sharma, M.R.,Booth, T.M.,Simpson, L.,Maslov, D.A.,Agrawal, R.K.
Structure of a mitochondrial ribosome with minimal RNA
Proc.Natl.Acad.Sci.USA, 106:9637-9642, 2009

PubMed Abstract: The Leishmania tarentolae mitochondrial ribosome (Lmr) is a minimal ribosomal RNA (rRNA)-containing ribosome. We have obtained a cryo-EM map of the Lmr. The map reveals several features that have not been seen in previously-determined structures of eubacterial or eukaryotic (cytoplasmic or organellar) ribosomes to our knowledge. Comparisons of the Lmr map with X-ray crystallographic and cryo-EM maps of the eubacterial ribosomes and a cryo-EM map of the mammalian mitochondrial ribosome show that (i) the overall structure of the Lmr is considerably more porous, (ii) the topology of the intersubunit space is significantly different, with fewer intersubunit bridges, but more tunnels, and (iii) several of the functionally-important rRNA regions, including the alpha-sarcin-ricin loop, have different relative positions within the structure. Furthermore, the major portions of the mRNA channel, the tRNA passage, and the nascent polypeptide exit tunnel contain Lmr-specific proteins, suggesting that the mechanisms for mRNA recruitment, tRNA interaction, and exiting of the nascent polypeptide in Lmr must differ markedly from the mechanisms deduced for ribosomes in other organisms. Our study identifies certain structural features that are characteristic solely of mitochondrial ribosomes and other features that are characteristic of both mitochondrial and chloroplast ribosomes (i.e., organellar ribosomes).

PubMed: 19497863
DOI: 10.1073/pnas.0901631106

実験手法

ELECTRON MICROSCOPY (14.1 Å)