3ITL

Crystal structure of Pseudomonas stutzeri L-rhamnose isomerase mutant D327N in complex with L-rhamnulose

3ITL の概要

• エントリーDOI: 10.2210/pdb3itl/pdb
• 関連するPDBエントリー: 2HCV 2I56 2I57 3ITO 3ITT 3ITV 3ITX 3ITY 3IUD 3IUH 3IUI
• 分子名称: L-rhamnose isomerase, MANGANESE (II) ION, 6-deoxy-beta-L-fructofuranose, ... (4 entities in total)
• 機能のキーワード: beta/alpha barrel, homo-tetramer, metal-binding protein, tim barrel, isomerase
• 由来する生物種: Pseudomonas stutzeri (Pseudomonas perfectomarina)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 193110.64

構造登録者

Yoshida, H.,Yamaji, M.,Ishii, T.,Izumori, K.,Kamitori, S. (登録日: 2009-08-28, 公開日: 2010-02-02, 最終更新日: 2023-11-01)

主引用文献

Yoshida, H.,Yamaji, M.,Ishii, T.,Izumori, K.,Kamitori, S.
Catalytic reaction mechanism of Pseudomonas stutzeri l-rhamnose isomerase deduced from X-ray structures
Febs J., 277:1045-1057, 2010

PubMed Abstract: L-Rhamnose isomerase (L-RhI) catalyzes the reversible isomerization of L-rhamnose to L-rhamnulose. Pseudomonas stutzeril-RhI, with a broad substrate specificity, can catalyze not only the isomerization of L-rhamnose, but also that between D-allose and D-psicose. For the aldose-ketose isomerization by L-RhI, a metal-mediated hydride-shift mechanism has been proposed, but the catalytic mechanism is still not entirely understood. To elucidate the entire reaction mechanism, the X-ray structures of P. stutzeril-RhI in an Mn(2+)-bound form, and of two inactive mutant forms of P. stutzeril-RhI (S329K and D327N) in a complex with substrate/product, were determined. The structure of the Mn(2+)-bound enzyme indicated that the catalytic site interconverts between two forms with the displacement of the metal ion to recognize both pyranose and furanose ring substrates. Solving the structures of S329K-substrates allowed us to examine the metal-mediated hydride-shift mechanism of L-RhI in detail. The structural analysis of D327N-substrates and additional modeling revealed Asp327 to be responsible for the ring opening of furanose, and a water molecule coordinating with the metal ion to be involved in the ring opening of pyranose.

PubMed: 20088877
DOI: 10.1111/j.1742-4658.2009.07548.x

実験手法

X-RAY DIFFRACTION (1.7 Å)