3ITI
Structure of bovine trypsin with the MAD triangle B3C
Summary for 3ITI
| Entry DOI | 10.2210/pdb3iti/pdb |
| Related | 3E3D 3E3S 3E3T 3GT3 3GT4 |
| Descriptor | Cationic trypsin, 5-amino-2,4,6-tribromobenzene-1,3-dicarboxylic acid, BENZAMIDINE, ... (6 entities in total) |
| Functional Keywords | phasing tool, 5-amino-2, 4, 6-tribromoisophthalic acid, b3c, mad triangle, i3c, magic triangle, digestion, disulfide bond, hydrolase, metal-binding, protease, secreted, serine protease, zymogen |
| Biological source | Bos taurus (bovine,cow,domestic cattle,domestic cow) |
| Cellular location | Secreted, extracellular space: P00760 |
| Total number of polymer chains | 1 |
| Total formula weight | 23937.80 |
| Authors | Beck, T.,da Cunha, C.E.,Sheldrick, G.M. (deposition date: 2009-08-28, release date: 2009-10-27, Last modification date: 2024-10-16) |
| Primary citation | Beck, T.,da Cunha, C.E.,Sheldrick, G.M. How to get the magic triangle and the MAD triangle into your protein crystal. Acta Crystallogr.,Sect.F, 65:1068-1070, 2009 Cited by PubMed Abstract: The magic triangle 5-amino-2,4,6-triiodoisophthalic acid (I3C) and the MAD triangle 5-amino-2,4,6-tribromoisophthalic acid (B3C) are two representatives of a novel class of compounds that combine heavy atoms for experimental phasing with functional groups for protein interactions. These compounds are readily available and provide easy access to experimental phasing. The preparation of stock solutions and the incorporation of the compounds into protein crystals are discussed. As an example of incorporation via cocrystallization, the incorporation of B3C into bovine trypsin, resulting in a single site with high occupancy, is described. PubMed: 19851024DOI: 10.1107/S1744309109036884 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.55 Å) |
Structure validation
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