3ITI

Structure of bovine trypsin with the MAD triangle B3C

3ITI の概要

• エントリーDOI: 10.2210/pdb3iti/pdb
• 関連するPDBエントリー: 3E3D 3E3S 3E3T 3GT3 3GT4
• 分子名称: Cationic trypsin, 5-amino-2,4,6-tribromobenzene-1,3-dicarboxylic acid, BENZAMIDINE, ... (6 entities in total)
• 機能のキーワード: phasing tool, 5-amino-2, 4, 6-tribromoisophthalic acid, b3c, mad triangle, i3c, magic triangle, digestion, disulfide bond, hydrolase, metal-binding, protease, secreted, serine protease, zymogen
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• 細胞内の位置: Secreted, extracellular space: P00760
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23937.80

構造登録者

Beck, T.,da Cunha, C.E.,Sheldrick, G.M. (登録日: 2009-08-28, 公開日: 2009-10-27, 最終更新日: 2024-10-16)

主引用文献

Beck, T.,da Cunha, C.E.,Sheldrick, G.M.
How to get the magic triangle and the MAD triangle into your protein crystal.
Acta Crystallogr.,Sect.F, 65:1068-1070, 2009

PubMed Abstract: The magic triangle 5-amino-2,4,6-triiodoisophthalic acid (I3C) and the MAD triangle 5-amino-2,4,6-tribromoisophthalic acid (B3C) are two representatives of a novel class of compounds that combine heavy atoms for experimental phasing with functional groups for protein interactions. These compounds are readily available and provide easy access to experimental phasing. The preparation of stock solutions and the incorporation of the compounds into protein crystals are discussed. As an example of incorporation via cocrystallization, the incorporation of B3C into bovine trypsin, resulting in a single site with high occupancy, is described.

PubMed: 19851024
DOI: 10.1107/S1744309109036884

実験手法

X-RAY DIFFRACTION (1.55 Å)