3ITE

The third adenylation domain of the fungal SidN non-ribosomal peptide synthetase

3ITE の概要

• エントリーDOI: 10.2210/pdb3ite/pdb
• 分子名称: SidN siderophore synthetase, SULFATE ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: ligase, non-ribosomal peptide synthesis, nrps, siderophore synthetase, sidna3, fungal, endophyte
• 由来する生物種: Neotyphodium lolii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 123433.40

構造登録者

Lee, T.V.,Lott, J.S.,Johnson, R.D.,Johnson, L.J.,Arcus, V.L. (登録日: 2009-08-28, 公開日: 2009-11-17, 最終更新日: 2024-11-06)

主引用文献

Lee, T.V.,Johnson, L.J.,Johnson, R.D.,Koulman, A.,Lane, G.A.,Lott, J.S.,Arcus, V.L.
Structure of a eukaryotic nonribosomal peptide synthetase adenylation domain that activates a large hydroxamate amino acid in siderophore biosynthesis
J.Biol.Chem., 285:2415-2427, 2010

PubMed Abstract: Nonribosomal peptide synthetases (NRPSs) are large, multidomain proteins that are involved in the biosynthesis of an array of secondary metabolites. We report the structure of the third adenylation domain from the siderophore-synthesizing NRPS, SidN, from the endophytic fungus Neotyphodium lolii. This is the first structure of a eukaryotic NRPS domain, and it reveals a large binding pocket required to accommodate the unusual amino acid substrate, N(delta)-cis-anhydromevalonyl-N(delta)-hydroxy-L-ornithine (cis-AMHO). The specific activation of cis-AMHO was confirmed biochemically, and an AMHO moiety was unambiguously identified as a component of the fungal siderophore using mass spectroscopy. The protein structure shows that the substrate binding pocket is defined by 17 amino acid residues, in contrast to both prokaryotic adenylation domains and to previous predictions based on modeling. Existing substrate prediction methods for NRPS adenylation domains fail for domains from eukaryotes due to the divergence of their signature sequences from those of prokaryotes. Thus, this new structure will provide a basis for improving prediction methods for eukaryotic NRPS enzymes that play important and diverse roles in the biology of fungi.

PubMed: 19923209
DOI: 10.1074/jbc.M109.071324

実験手法

X-RAY DIFFRACTION (2 Å)