3IQR

SAM-I riboswitch from T. tencongensis variant A94G bound with SAM

3IQR の概要

• エントリーDOI: 10.2210/pdb3iqr/pdb
• 関連するPDBエントリー: 2GIS 3IQN 3IQP
• 分子名称: SAM-I riboswitch, BARIUM ION, S-ADENOSYLMETHIONINE, ... (4 entities in total)
• 機能のキーワード: riboswitch, sam, kink-turn, psuedoknot, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32203.70

構造登録者

Montange, R.K.,Batey, R.T. (登録日: 2009-08-20, 公開日: 2010-08-04, 最終更新日: 2023-09-06)

主引用文献

Stoddard, C.D.,Montange, R.K.,Hennelly, S.P.,Rambo, R.P.,Sanbonmatsu, K.Y.,Batey, R.T.
Free state conformational sampling of the SAM-I riboswitch aptamer domain.
Structure, 18:787-797, 2010

PubMed Abstract: Riboswitches are highly structured elements residing in the 5' untranslated region of messenger RNAs that specifically bind cellular metabolites to alter gene expression. While there are many structures of ligand-bound riboswitches that reveal details of bimolecular recognition, their unliganded structures remain poorly characterized. Characterizing the molecular details of the unliganded state is crucial for understanding the riboswitch's mechanism of action because it is this state that actively interrogates the cellular environment and helps direct the regulatory outcome. To develop a detailed description of the ligand-free form of an S-adenosylmethionine binding riboswitch at the local and global levels, we have employed a series of biochemical, biophysical, and computational methods. Our data reveal that the ligand binding domain adopts an ensemble of states that minimizes the energy barrier between the free and bound states to establish an efficient decision making branchpoint in the regulatory process.

PubMed: 20637415
DOI: 10.1016/j.str.2010.04.006

実験手法

X-RAY DIFFRACTION (2.55 Å)