3IPZ

Crystal structure of Arabidopsis monothiol glutaredoxin AtGRXcp

3IPZ の概要

• エントリーDOI: 10.2210/pdb3ipz/pdb
• 分子名称: Monothiol glutaredoxin-S14, chloroplastic (2 entities in total)
• 機能のキーワード: glutaredoxin, monothiol, chloroplast, electron transport, plastid, redox-active center, transit peptide, transport, oxidoreductase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress)
• 細胞内の位置: Plastid, chloroplast: Q84Y95
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12354.15

構造登録者

Li, L.,Cheng, N.,Wang, X. (登録日: 2009-08-18, 公開日: 2010-06-16, 最終更新日: 2024-11-06)

主引用文献

Li, L.,Cheng, N.,Hirschi, K.D.,Wang, X.
Structure of Arabidopsis chloroplastic monothiol glutaredoxin AtGRXcp.
Acta Crystallogr.,Sect.D, 66:725-732, 2010

PubMed Abstract: Monothiol glutaredoxins (Grxs) play important roles in maintaining redox homeostasis in living cells and are conserved across species. Arabidopsis thaliana monothiol glutaredoxin AtGRXcp is critical for protection from oxidative stress in chloroplasts. The crystal structure of AtGRXcp has been determined at 2.4 A resolution. AtGRXcp has a glutaredoxin/thioredoxin-like fold with distinct structural features that differ from those of dithiol Grxs. The structure reveals that the putative active-site motif CGFS is well defined and is located on the molecular surface and that a long groove extends to both sides of the catalytic Cys97. Structural comparison and molecular modeling suggest that glutathione can bind in this groove and form extensive interactions with conserved charged residues including Lys89, Arg126 and Asp152. Further comparative studies reveal that a unique loop with five additional residues adjacent to the active-site motif may be a key structural feature of monothiol Grxs and may influence their function. This study provides the first structural information on plant CGFS-type monothiol Grxs, allowing a better understanding of the redox-regulation mechanism mediated by these plant Grxs.

PubMed: 20516625
DOI: 10.1107/S0907444910013119

実験手法

X-RAY DIFFRACTION (2.4 Å)