3IK5

SIVmac239 Nef in complex with TCR zeta ITAM 1 polypeptide (A63-R80)

3IK5 の概要

• エントリーDOI: 10.2210/pdb3ik5/pdb
• 分子名称: Protein Nef, T-cell surface glycoprotein CD3 zeta chain (3 entities in total)
• 機能のキーワード: protein-protein complex, cell membrane, lipoprotein, membrane, myristate, viral immunoevasion, virulence, disulfide bond, host-virus interaction, phosphoprotein, receptor, transmembrane, viral protein-antiviral protein complex, viral protein-signaling protein complex, viral protein/signaling protein
• 由来する生物種: Simian immunodeficiency virus (SIV-cpz); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P20963
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 37732.04

構造登録者

Kim, W.M.,Sigalov, A.B.,Stern, L.J. (登録日: 2009-08-05, 公開日: 2010-02-02, 最終更新日: 2024-02-21)

主引用文献

Kim, W.M.,Sigalov, A.B.,Stern, L.J.
Pseudo-merohedral twinning and noncrystallographic symmetry in orthorhombic crystals of SIVmac239 Nef core domain bound to different-length TCRzeta fragments.
Acta Crystallogr.,Sect.D, 66:163-175, 2010

PubMed Abstract: HIV/SIV Nef mediates many cellular processes through interactions with various cytoplasmic and membrane-associated host proteins, including the signalling zeta subunit of the T-cell receptor (TCRzeta). Here, the crystallization strategy, methods and refinement procedures used to solve the structures of the core domain of the SIVmac239 isolate of Nef (Nef(core)) in complex with two different TCRzeta fragments are described. The structure of SIVmac239 Nef(core) bound to the longer TCRzeta polypeptide (Leu51-Asp93) was determined to 3.7 A resolution (R(work) = 28.7%) in the tetragonal space group P4(3)2(1)2. The structure of SIVmac239 Nef(core) in complex with the shorter TCRzeta polypeptide (Ala63-Arg80) was determined to 2.05 A resolution (R(work) = 17.0%), but only after the detection of nearly perfect pseudo-merohedral crystal twinning and proper assignment of the orthorhombic space group P2(1)2(1)2(1). The reduction in crystal space-group symmetry induced by the truncated TCRzeta polypeptide appears to be caused by the rearrangement of crystal-contact hydrogen-bonding networks and the substitution of crystallographic symmetry operations by similar noncrystallographic symmetry (NCS) operations. The combination of NCS rotations that were nearly parallel to the twin operation (k, h, -l) and a and b unit-cell parameters that were nearly identical predisposed the P2(1)2(1)2(1) crystal form to pseudo-merohedral twinning.

PubMed: 20124696
DOI: 10.1107/S090744490904880X

実験手法

X-RAY DIFFRACTION (2.05 Å)