3IJW

Crystal structure of BA2930 in complex with CoA

3IJW の概要

• エントリーDOI: 10.2210/pdb3ijw/pdb
• 分子名称: Aminoglycoside N3-acetyltransferase, ACETYL COENZYME *A, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: anthrax, coa, transferase, acyltransferase, structural genomics, center for structural genomics of infectious diseases, csgid
• 由来する生物種: Bacillus anthracis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62424.15

構造登録者

Klimecka, M.M.,Chruszcz, M.,Skarina, T.,Onopryienko, O.,Cymborowski, M.,Savchenko, A.,Edwards, A.,Anderson, W.,Minor, W.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2009-08-05, 公開日: 2009-10-13, 最終更新日: 2023-11-22)

主引用文献

Klimecka, M.M.,Chruszcz, M.,Font, J.,Skarina, T.,Shumilin, I.,Onopryienko, O.,Porebski, P.J.,Cymborowski, M.,Zimmerman, M.D.,Hasseman, J.,Glomski, I.J.,Lebioda, L.,Savchenko, A.,Edwards, A.,Minor, W.
Structural Analysis of a Putative Aminoglycoside N-Acetyltransferase from Bacillus anthracis.
J.Mol.Biol., 410:411-423, 2011

PubMed Abstract: For the last decade, worldwide efforts for the treatment of anthrax infection have focused on developing effective vaccines. Patients that are already infected are still treated traditionally using different types of standard antimicrobial agents. The most popular are antibiotics such as tetracyclines and fluoroquinolones. While aminoglycosides appear to be less effective antimicrobial agents than other antibiotics, synthetic aminoglycosides have been shown to act as potent inhibitors of anthrax lethal factor and may have potential application as antitoxins. Here, we present a structural analysis of the BA2930 protein, a putative aminoglycoside acetyltransferase, which may be a component of the bacterium's aminoglycoside resistance mechanism. The determined structures revealed details of a fold characteristic only for one other protein structure in the Protein Data Bank, namely, YokD from Bacillus subtilis. Both BA2930 and YokD are members of the Antibiotic_NAT superfamily (PF02522). Sequential and structural analyses showed that residues conserved throughout the Antibiotic_NAT superfamily are responsible for the binding of the cofactor acetyl coenzyme A. The interaction of BA2930 with cofactors was characterized by both crystallographic and binding studies.

PubMed: 21601576
DOI: 10.1016/j.jmb.2011.04.076

実験手法

X-RAY DIFFRACTION (1.9 Å)