3IJ9

Directed 'in situ' Elongation as a Strategy to Characterize the Covalent Glycosyl-Enzyme Catalytic Intermediate of Human Pancreatic a-Amylase

3IJ9 の概要

• エントリーDOI: 10.2210/pdb3ij9/pdb
• 関連するPDBエントリー: 3IJ7 3IJ8
• 分子名称: Pancreatic alpha-amylase, beta-D-glucopyranose-(1-2)-5-fluoro-alpha-L-idopyranose, (2R,3S,4R,5R,6R)-2,6-difluoro-2-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol, ... (6 entities in total)
• 機能のキーワード: amylase, covalent intermediate, hydrolytic cleavage, catalysis, inhibitor synthesis, enzyme kinetics, human digestion, diabetes, obesity, carbohydrate metabolism, chloride, disulfide bond, glycoprotein, glycosidase, hydrolase, metal-binding, pyrrolidone carboxylic acid, secreted
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56767.40

構造登録者

Li, C.,Zhang, R.,Withers, S.G.,Brayer, G.D. (登録日: 2009-08-04, 公開日: 2009-10-27, 最終更新日: 2020-07-29)

• 主引用文献: Zhang, R.,Li, C.,Williams, L.K.,Rempel, B.P.,Brayer, G.D.,Withers, S.G.; Directed "in situ" inhibitor elongation as a strategy to structurally characterize the covalent glycosyl-enzyme intermediate of human pancreatic alpha-amylase; Biochemistry, 48:10752-10764, 2009; PubMed: 19803533; DOI: 10.1021/bi901400p

実験手法

X-RAY DIFFRACTION (1.85 Å)