3ICW

Structure of a Circular Permutation on Lipase B from Candida Antartica with Bound Suicide Inhibitor

3ICW の概要

• エントリーDOI: 10.2210/pdb3icw/pdb
• 関連するPDBエントリー: 3ICV
• 分子名称: Lipase B, 2-acetamido-2-deoxy-beta-D-glucopyranose, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: circular permutation, suicide inhibition, hydrolase, cleavage on pair of basic residues, disulfide bond, glycoprotein, lipid degradation, zymogen
• 由来する生物種: Candida antarctica (Yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33616.64

構造登録者

Horton, J.R.,Qian, Z.,Jia, D.,Lutz, S.A.,Cheng, X. (登録日: 2009-07-18, 公開日: 2009-10-06, 最終更新日: 2024-11-20)

主引用文献

Qian, Z.,Horton, J.R.,Cheng, X.,Lutz, S.
Structural redesign of lipase B from Candida antarctica by circular permutation and incremental truncation.
J.Mol.Biol., 393:191-201, 2009

PubMed Abstract: Circular permutation of Candida antarctica lipase B yields several enzyme variants with substantially increased catalytic activity. To better understand the structural and functional consequences of protein termini reorganization, we have applied protein engineering and x-ray crystallography to cp283, one of the most active hydrolase variants. Our initial investigation has focused on the role of an extended surface loop, created by linking the native N- and C-termini, on protein integrity. Incremental truncation of the loop partially compensates for observed losses in secondary structure and the permutants' temperature of unfolding. Unexpectedly, the improvements are accompanied by quaternary-structure changes from monomer to dimer. The crystal structures of one truncated variant (cp283 Delta 7) in the apo-form determined at 1.49 A resolution and with a bound phosphonate inhibitor at 1.69 A resolution confirmed the formation of a homodimer by swapping of the enzyme's 35-residue N-terminal region. Separately, the new protein termini at amino acid positions 282/283 convert the narrow access tunnel to the catalytic triad into a broad crevice for accelerated substrate entry and product exit while preserving the native active-site topology for optimal catalytic turnover.

PubMed: 19683009
DOI: 10.1016/j.jmb.2009.08.008

実験手法

X-RAY DIFFRACTION (1.69 Å)