3IBF

Crystal structure of unliganded caspase-7

3IBF の概要

• エントリーDOI: 10.2210/pdb3ibf/pdb
• 関連するPDBエントリー: 3IBC
• 分子名称: Caspase-7 (3 entities in total)
• 機能のキーワード: protein structure, alternative splicing, apoptosis, cytoplasm, hydrolase, polymorphism, protease, thiol protease, zymogen
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: P55210 P55210
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 61834.92

構造登録者

Agniswamy, J. (登録日: 2009-07-15, 公開日: 2009-09-01, 最終更新日: 2023-09-06)

主引用文献

Agniswamy, J.,Fang, B.,Weber, I.T.
Conformational similarity in the activation of caspase-3 and -7 revealed by the unliganded and inhibited structures of caspase-7.
Apoptosis, 14:1135-1144, 2009

PubMed Abstract: Caspase-mediated apoptosis has important roles in normal cell differentiation and aging and in many diseases including cancer, neuromuscular disorders and neurodegenerative diseases. Therefore, modulation of caspase activity and conformational states is of therapeutic importance. We report crystal structures of a new unliganded conformation of caspase-7 and the inhibited caspase-7 with the tetrapeptide Ac-YVAD-Cho. Different conformational states and mechanisms for substrate recognition have been proposed based on unliganded structures of the redundant apoptotic executioner caspase-3 and -7. The current study shows that the executioner caspase-3 and -7 have similar conformations for the unliganded active site as well as the inhibitor-bound active site. The new unliganded caspase-7 structure exhibits the tyrosine flipping mechanism in which the Tyr230 has rotated to block entry to the S2 binding site similar to the active site conformation of unliganded caspase-3. The inhibited structure of caspase-7/YVAD shows that the P4 Tyr binds the S4 region specific to polar residues at the expense of a main chain hydrogen bond between the P4 amide and carbonyl oxygen of caspase-7 Gln 276, which is similar to the caspase-3 complex. This new knowledge of the structures and conformational states of unliganded and inhibited caspases will be important for the design of drugs to modulate caspase activity and apoptosis.

PubMed: 19655253
DOI: 10.1007/s10495-009-0388-9

実験手法

X-RAY DIFFRACTION (2.5 Å)