3I9Z

Crystal structure of a metallochaperone with a trinuclear Cu(I) cluster

3I9Z の概要

• エントリーDOI: 10.2210/pdb3i9z/pdb
• 関連するPDBエントリー: 2QIF
• 分子名称: Copper chaperone copZ, COPPER (I) ION (3 entities in total)
• 機能のキーワード: chaperone, copper, cytoplasm, metal-binding
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cytoplasm: O32221
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7409.72

構造登録者

Hearnshaw, S.J.,Zhou, L.,Le Brun, N.E.,Hemmings, A.M. (登録日: 2009-07-13, 公開日: 2009-11-17, 最終更新日: 2024-02-21)

主引用文献

Singleton, C.,Hearnshaw, S.,Zhou, L.,Le Brun, N.E.,Hemmings, A.M.
Mechanistic insights into Cu(I) cluster transfer between the chaperone CopZ and its cognate Cu(I)-transporting P-type ATPase, CopA.
Biochem.J., 424:347-356, 2009

PubMed Abstract: Multinuclear Cu(I) clusters are common in nature, but little is known about their formation or transfer between proteins. CopZ and CopA from Bacillus subtilis, which are involved in a copper-efflux pathway, both readily accommodate multinuclear Cu(I) clusters. Using the luminescence properties of a multinuclear Cu(I)-bound form of the two N-terminal soluble domains of CopA (CopAab) we have investigated the thermodynamic and kinetic properties of cluster formation and loss. We demonstrate that Cu(I)-bound forms of dimeric CopZ containing more than one Cu(I) per CopZ monomer can transfer Cu(I) to apo-CopAab, leading to the formation of luminescent dimeric CopAab. Kinetic studies demonstrated that transfer is a first-order process and that the rate-determining steps for transfer from CopZ to CopAab and vice versa are different processes. The rate of formation of luminescent CopAab via transfer of Cu(I) from CopZ was more rapid than that observed when Cu(I) was added 'directly' from solution or in complex with a cysteine variant of CopZ, indicating that transfer occurs via a transient protein-protein complex. Such a complex would probably require the interaction of at least one domain of CopAab with the CopZ dimer. Insight into how such a complex might form is provided by the high resolution crystal structure of Cu3(CopZ)3, a thus far unique trimeric form of CopZ containing a trinuclear Cu(I) cluster. Modelling studies showed that one of the CopZ monomers can be substituted for either domain of CopAab, resulting in a heterotrimer, thus providing a model for a 'trapped' copper exchange complex.

PubMed: 19751213
DOI: 10.1042/BJ20091079

実験手法

X-RAY DIFFRACTION (1.9 Å)