3I4R

Nup107(aa658-925)/Nup133(aa517-1156) complex, H.sapiens

3I4R の概要

• エントリーDOI: 10.2210/pdb3i4r/pdb
• 分子名称: Nuclear pore complex protein Nup107, Nuclear pore complex protein Nup133 (2 entities in total)
• 機能のキーワード: protein transport, structural protein, kinetochore, mrna transport, nuclear pore complex, nucleus, phosphoprotein, translocation, transport, polymorphism
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus, nuclear pore complex: P57740 Q8WUM0
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 106086.68

構造登録者

Whittle, J.R.R.,Schwartz, T.U. (登録日: 2009-07-02, 公開日: 2009-08-11, 最終更新日: 2024-02-21)

主引用文献

Whittle, J.R.,Schwartz, T.U.
Architectural nucleoporins Nup157/170 and Nup133 are structurally related and descend from a second ancestral element.
J.Biol.Chem., 284:28442-28452, 2009

PubMed Abstract: The nuclear pore complex (NPC) constitutes one of the largest protein assemblies in the eukaryotic cell and forms the exclusive gateway to the nucleus. The stable, approximately 15-20-MDa scaffold ring of the NPC is built from two multiprotein complexes arranged around a central 8-fold axis. Here we present crystal structures of two large architectural units, yNup170(979-1502) and hNup107(658-925) x hNup133(517-1156), each a constituent of one of the two multiprotein complexes. Conservation of domain arrangement and of tertiary structure suggests that Nup157/170 and Nup133 derived from a common ancestor. Together with the previously established ancestral coatomer element (ACE1), these two elements constitute the major alpha-helical building blocks of the NPC scaffold and define its branched, lattice-like architecture, similar to vesicle coats like COPII. We hypothesize that the extant NPC evolved early during eukaryotic evolution from a rudimentary structure composed of several identical copies of a few ancestral elements, later diversified and specified by gene duplication.

PubMed: 19674973
DOI: 10.1074/jbc.M109.023580

実験手法

X-RAY DIFFRACTION (3.53 Å)