3I3R

X-ray structure dihydrofolate reductase/thymidylate synthase from babesia bovis at 2.35A resolution

3I3R の概要

• エントリーDOI: 10.2210/pdb3i3r/pdb
• 分子名称: Dihydrofolate reductase/thymidylate synthase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: ssgcid, babesia bovis, dihydrofolate reductase, thymidylate synthase, methyltransferase, transferase, structural genomics, seattle structural genomics center for infectious disease
• 由来する生物種: Babesia bovis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 116638.22

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID),Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2009-06-30, 公開日: 2009-08-18, 最終更新日: 2023-09-06)

主引用文献

Begley, D.W.,Edwards, T.E.,Raymond, A.C.,Smith, E.R.,Hartley, R.C.,Abendroth, J.,Sankaran, B.,Lorimer, D.D.,Myler, P.J.,Staker, B.L.,Stewart, L.J.
Inhibitor-bound complexes of dihydrofolate reductase-thymidylate synthase from Babesia bovis.
Acta Crystallogr.,Sect.F, 67:1070-1077, 2011

PubMed Abstract: Babesiosis is a tick-borne disease caused by eukaryotic Babesia parasites which are morphologically similar to Plasmodium falciparum, the causative agent of malaria in humans. Like Plasmodium, different species of Babesia are tuned to infect different mammalian hosts, including rats, dogs, horses and cattle. Most species of Plasmodium and Babesia possess an essential bifunctional enzyme for nucleotide synthesis and folate metabolism: dihydrofolate reductase-thymidylate synthase. Although thymidylate synthase is highly conserved across organisms, the bifunctional form of this enzyme is relatively uncommon in nature. The structural characterization of dihydrofolate reductase-thymidylate synthase in Babesia bovis, the causative agent of babesiosis in livestock cattle, is reported here. The apo state is compared with structures that contain dUMP, NADP and two different antifolate inhibitors: pemetrexed and raltitrexed. The complexes reveal modes of binding similar to that seen in drug-resistant malaria strains and point to the utility of applying structural studies with proven cancer chemotherapies towards infectious disease research.

PubMed: 21904052
DOI: 10.1107/S1744309111029009

実験手法

X-RAY DIFFRACTION (2.35 Å)