3I2Z

Structure of cold shock protein E from Salmonella typhimurium

3I2Z の概要

• エントリーDOI: 10.2210/pdb3i2z/pdb
• 関連するPDBエントリー: 1C9O 1CSP 1MJC 2ES2 2HAX
• 分子名称: RNA chaperone, negative regulator of cspA transcription (2 entities in total)
• 機能のキーワード: beta barrel, dna binding protein/transcription, cytoplasm, gene regulation
• 由来する生物種: Salmonella typhimurium
• 細胞内の位置: Cytoplasm (By similarity): Q7CQZ5
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15371.19

構造登録者

Morgan, H.P.,McNae, I.,Wear, M.A.,Gallagher, M.,Walkinshaw, M.D. (登録日: 2009-06-30, 公開日: 2009-12-22, 最終更新日: 2023-11-01)

主引用文献

Morgan, H.P.,Wear, M.A.,McNae, I.,Gallagher, M.P.,Walkinshaw, M.D.
Crystallization and X-ray structure of cold-shock protein E from Salmonella typhimurium
Acta Crystallogr.,Sect.F, 65:1240-1245, 2009

PubMed Abstract: In prokaryotic organisms, cold shock triggers the production of a small highly conserved family of cold-shock proteins (CSPs). CSPs have been well studied structurally and functionally in Escherichia coli and Bacillus subtilis, but Salmonella typhimurium CSPs remain relatively uncharacterized. In S. typhimurium, six homologous CSPs have been identified: StCspA-E and StCspH. The crystal structure of cold-shock protein E from S. typhimurium (StCspE) has been determined at 1.1 A resolution and has an R factor of 0.203 after refinement. The three-dimensional structure is similar to those of previously determined CSPs and is composed of five antiparallel beta-strands forming a classic OB fold/five-stranded beta-barrel. This first structure of a CSP from S. typhimurium provides new insight into the cold-shock response of this bacterium.

PubMed: 20054119
DOI: 10.1107/S1744309109033788

実験手法

X-RAY DIFFRACTION (1.1 Å)