3HZY

Crystal structure of S73-2 antibody in complex with antigen Kdo(2.4)Kdo(2.4)Kdo

3HZY の概要

• エントリーDOI: 10.2210/pdb3hzy/pdb
• 関連するPDBエントリー: 3HZK 3HZM 3HZV 3I02
• 分子名称: S73-2 Fab (IgG1k) light chain, S73-2 Fab (IgG1k) heavy chain, 3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid-(2-4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid-(2-4)-prop-2-en-1-yl 3-deoxy-alpha-D-manno-oct-2-ulopyranosidonic acid, ... (6 entities in total)
• 機能のキーワード: antibody, fab, igg, carbohydrate, immune system
• 由来する生物種: Mus musculus (mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49671.13

構造登録者

Brooks, C.L.,Muller-Loennies, S.,Borisova, S.N.,Brade, L.,Kosma, P.,Hirama, T.,MacKenzie, C.R.,Brade, H.,Evans, S.V. (登録日: 2009-06-24, 公開日: 2010-01-12, 最終更新日: 2024-11-06)

主引用文献

Brooks, C.L.,Muller-Loennies, S.,Borisova, S.N.,Brade, L.,Kosma, P.,Hirama, T.,Mackenzie, C.R.,Brade, H.,Evans, S.V.
Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition
Biochemistry, 49:570-581, 2010

PubMed Abstract: The structures of antigen-binding fragments from two related monoclonal antibodies have been determined to high resolution in the presence of several carbohydrate antigens raised against chlamydial lipopolysaccharide. With the exception of CDR H3, antibodies S54-10 and S73-2 are both derived from the same set of germline gene segments as the previously reported structures S25-2 and S45-18. Despite this similarity, the antibodies differ in specificity and the mechanism by which they recognize their cognate antigen. S54-10 uses an unrelated CDR H3 to recognize its antigen in a fashion analogous to S45-18; however, S73-2 recognizes the same antigen as S45-18 and S54-10 in a wholly unrelated manner. Together, these antibody-antigen structures provide snapshots into how the immune system uses the same set of inherited germline gene segments to generate multiple possible specificities that allow for differential recognition of epitopes and how unrelated CDR H3 sequences can result in convergent binding of clinically relevant bacterial antigens.

PubMed: 20000757
DOI: 10.1021/bi9011308

実験手法

X-RAY DIFFRACTION (2.1 Å)