3HTS

HEAT SHOCK TRANSCRIPTION FACTOR/DNA COMPLEX

3HTS の概要

• エントリーDOI: 10.2210/pdb3hts/pdb
• 分子名称: 5'-D(*GP*GP*TP*TP*CP*TP*AP*GP*AP*AP*CP*C)-3', HEAT SHOCK TRANSCRIPTION FACTOR, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: transcription regulation, dna-binding protein, complex (winged helix_turn_ helix-dna), transcription-dna complex, transcription/dna
• 由来する生物種: Kluyveromyces lactis; 詳細
• 細胞内の位置: Nucleus: P22121
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15695.91

構造登録者

Littlefield, O.,Nelson, H.C.M. (登録日: 1998-11-16, 公開日: 1999-04-29, 最終更新日: 2023-09-06)

主引用文献

Littlefield, O.,Nelson, H.C.
A new use for the 'wing' of the 'winged' helix-turn-helix motif in the HSF-DNA cocrystal.
Nat.Struct.Biol., 6:464-470, 1999

PubMed Abstract: The 1.75 A crystal structure of the Kluyveromyces lactis heat shock transcription factor (HSF) DNA-binding domain (DBD) complexed with DNA reveals a protein-DNA interface with few direct major groove contacts and a number of phosphate backbone contacts that are primarily water-mediated interactions. The DBD, a 'winged' helix-turn-helix protein, displays a novel mode of binding in that the 'wing' does not contact DNA like all others of that class. Instead, the monomeric DBD, which crystallized as a symmetric dimer to a pair of nGAAn inverted repeats, uses the 'wing' to form part of the protein-protein contacts. This dimer interface is likely important for increasing the DNA-binding specificity and affinity of the trimeric form of HSF, as well as for increasing cooperativity between adjacent trimers.

PubMed: 10331875
DOI: 10.1038/8269

実験手法

X-RAY DIFFRACTION (1.75 Å)