3HRI

Histidyl-tRNA synthetase (apo) from Trypanosoma brucei

3HRI の概要

• エントリーDOI: 10.2210/pdb3hri/pdb
• 関連するPDBエントリー: 3HRJ 3HRK
• 分子名称: Histidyl-tRNA synthetase (1 entity in total)
• 機能のキーワード: apo trna-ligase, aminoacyl-trna synthetase, ligase, structural genomics, medical structural genomics of pathogenic protozoa, msgpp
• 由来する生物種: Trypanosoma brucei
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 307245.73

構造登録者

Arakaki, T.L.,Merritt, E.A.,Larson, E.T.,Medical Structural Genomics of Pathogenic Protozoa (MSGPP) (登録日: 2009-06-09, 公開日: 2009-12-01, 最終更新日: 2024-04-03)

主引用文献

Merritt, E.A.,Arakaki, T.L.,Gillespie, J.R.,Larson, E.T.,Kelley, A.,Mueller, N.,Napuli, A.J.,Kim, J.,Zhang, L.,Verlinde, C.L.,Fan, E.,Zucker, F.,Buckner, F.S.,van Voorhis, W.C.,Hol, W.G.
Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs.
J.Mol.Biol., 397:481-494, 2010

PubMed Abstract: Crystal structures of histidyl-tRNA synthetase (HisRS) from the eukaryotic parasites Trypanosoma brucei and Trypanosoma cruzi provide a first structural view of a eukaryotic form of this enzyme and reveal differences from bacterial homologs. HisRSs in general contain an extra domain inserted between conserved motifs 2 and 3 of the Class II aminoacyl-tRNA synthetase catalytic core. The current structures show that the three-dimensional topology of this domain is very different in bacterial and archaeal/eukaryotic forms of the enzyme. Comparison of apo and histidine-bound trypanosomal structures indicates substantial active-site rearrangement upon histidine binding but relatively little subsequent rearrangement after reaction of histidine with ATP to form the enzyme's first reaction product, histidyladenylate. The specific residues involved in forming the binding pocket for the adenine moiety differ substantially both from the previously characterized binding site in bacterial structures and from the homologous residues in human HisRSs. The essentiality of the single HisRS gene in T. brucei is shown by a severe depression of parasite growth rate that results from even partial suppression of expression by RNA interference.

PubMed: 20132829
DOI: 10.1016/j.jmb.2010.01.051

実験手法

X-RAY DIFFRACTION (2.85 Å)