3HQ2

BsuCP Crystal Structure

3HQ2 の概要

• エントリーDOI: 10.2210/pdb3hq2/pdb
• 分子名称: Bacillus subtilis M32 carboxypeptidase, ZINC ION, PHOSPHATE ION, ... (6 entities in total)
• 機能のキーワード: hydrolase, metal-binding, metalloprotease, protease, zinc
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 117407.54

構造登録者

Lee, M.M.,Isaza, C.E.,White, J.D.,Chen, R.P.-Y.,Liang, G.F.-C.,He, H.T.-F.,Chan, S.I.,Chan, M.K. (登録日: 2009-06-05, 公開日: 2009-06-30, 最終更新日: 2024-02-21)

主引用文献

Lee, M.M.,Isaza, C.E.,White, J.D.,Chen, R.P.,Liang, G.F.,He, H.T.,Chan, S.I.,Chan, M.K.
Insight into the substrate length restriction of M32 carboxypeptidases: Characterization of two distinct subfamilies.
Proteins, 77:647-657, 2009

PubMed Abstract: M32 carboxypeptidases are a distinct family of HEXXH metalloproteases whose structures exhibit a narrow substrate groove that is blocked at one end. Structural alignments with other HEXXH metalloprotease-peptide complexes suggested an orientation in which the substrate is directed towards the back of the groove. This led us to hypothesize, and subsequently confirm that the maximum substrate length for M32 carboxypeptidases is restricted. Structural and sequence analyses implicate a highly conserved Arg at the back of the groove as being critical for this length restriction. However, the Thermus thermophilus and Bacillus subtilis M32 members lack this conserved Arg. Herein, we present the biochemical and structural characterization of these two proteins. Our findings support the important role of the conserved Arg in maintaining the length restriction, and reveal a proline-rich loop as an alternate blocking strategy. Based on our results, we propose that M32 carboxypeptidases from Bacilli belong to a separate subfamily.

PubMed: 19544567
DOI: 10.1002/prot.22478

実験手法

X-RAY DIFFRACTION (2.9 Å)