3HNE

Crystal structure of human ribonucleotide reductase 1 bound to the effectors TTP and ATP

3HNE の概要

• エントリーDOI: 10.2210/pdb3hne/pdb
• 関連するPDBエントリー: 3HNC 3HND 3HNF
• 分子名称: Ribonucleoside-diphosphate reductase large subunit, THYMIDINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: oxidoreductase, ribonucleotide reductase, allosteric enzyme, atp-binding, dna replication, nucleotide-binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P23921
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 182503.16

構造登録者

Fairman, J.W.,Wijerathna, S.R.,Xu, H.,Dealwis, C.G. (登録日: 2009-05-31, 公開日: 2011-02-23, 最終更新日: 2023-09-06)

主引用文献

Fairman, J.W.,Wijerathna, S.R.,Ahmad, M.F.,Xu, H.,Nakano, R.,Jha, S.,Prendergast, J.,Welin, R.M.,Flodin, S.,Roos, A.,Nordlund, P.,Li, Z.,Walz, T.,Dealwis, C.G.
Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization.
Nat.Struct.Mol.Biol., 18:316-322, 2011

PubMed Abstract: Ribonucleotide reductase (RR) is an α(n)β(n) (RR1-RR2) complex that maintains balanced dNTP pools by reducing NDPs to dNDPs. RR1 is the catalytic subunit, and RR2 houses the free radical required for catalysis. RR is allosterically regulated by its activator ATP and its inhibitor dATP, which regulate RR activity by inducing oligomerization of RR1. Here, we report the first X-ray structures of human RR1 bound to TTP alone, dATP alone, TTP-GDP, TTP-ATP, and TTP-dATP. These structures provide insights into regulation of RR by ATP or dATP. At physiological dATP concentrations, RR1 forms inactive hexamers. We determined the first X-ray structure of the RR1-dATP hexamer and used single-particle electron microscopy to visualize the α(6)-ββ'-dATP holocomplex. Site-directed mutagenesis and functional assays confirm that hexamerization is a prerequisite for inhibition by dATP. Our data indicate a mechanism for regulating RR activity by dATP-induced oligomerization.

PubMed: 21336276
DOI: 10.1038/nsmb.2007

実験手法

X-RAY DIFFRACTION (3.11 Å)