3HHZ

Complex of the vesicular stomatitis virus nucleocapsid and the nucleocapsid-binding domain of the phosphoprotein

3HHZ の概要

• エントリーDOI: 10.2210/pdb3hhz/pdb
• 関連するPDBエントリー: 3HHW
• 分子名称: Phosphoprotein, Nucleoprotein, RNA (45-MER) (3 entities in total)
• 機能のキーワード: protein, rna, template, replication, negative strand rna virus, chaperone, phosphoprotein, rna replication, virion, ribonucleoprotein, rna-binding, viral nucleoprotein, viral protein, viral protein-rna complex, viral protein/rna
• 由来する生物種: Vesicular stomatitis Indiana virus (VSIV); 詳細
• 細胞内の位置: Virion: P04880 Q77E03
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 299362.50

構造登録者

Green, T.J.,Luo, M. (登録日: 2009-05-18, 公開日: 2009-07-28, 最終更新日: 2023-09-06)

主引用文献

Green, T.J.,Luo, M.
Structure of the vesicular stomatitis virus nucleocapsid in complex with the nucleocapsid-binding domain of the small polymerase cofactor, P.
Proc.Natl.Acad.Sci.USA, 106:11713-11718, 2009

PubMed Abstract: The negative-strand RNA viruses (NSRVs) are unique because their nucleocapsid, not the naked RNA, is the active template for transcription and replication. The viral polymerase of nonsegmented NSRVs contains a large polymerase catalytic subunit (L) and a nonenzymatic cofactor, the phosphoprotein (P). Insight into how P delivers the polymerase complex to the nucleocapsid has long been pursued by reverse genetics and biochemical approaches. Here, we present the X-ray crystal structure of the C-terminal domain of P of vesicular stomatitis virus, a prototypic nonsegmented NSRV, bound to nucleocapsid-like particles. P binds primarily to the C-terminal lobe of 2 adjacent N proteins within the nucleocapsid. This binding mode is exclusive to the nucleocapsid, not the nucleocapsid (N) protein in other existing forms. Localization of phosphorylation sites within P and their proximity to the RNA cavity give insight into how the L protein might be oriented to access the RNA template.

PubMed: 19571006
DOI: 10.1073/pnas.0903228106

実験手法

X-RAY DIFFRACTION (3.5 Å)