3HHF

Structure of CrgA regulatory domain, a LysR-type transcriptional regulator from Neisseria meningitidis.

3HHF の概要

• エントリーDOI: 10.2210/pdb3hhf/pdb
• 関連するPDBエントリー: 3HHG
• 分子名称: Transcriptional regulator, LysR family, CHLORIDE ION (3 entities in total)
• 機能のキーワード: neisseria meningitidis, transcription factor, lysr, structural genomics, oxford protein production facility, oppf, dna-binding, transcription, transcription regulation, transcription regulator
• 由来する生物種: Neisseria meningitidis serogroup B
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46949.65

構造登録者

Sainsbury, S.,Ren, J.,Owens, R.J.,Stuart, D.I.,Oxford Protein Production Facility (OPPF) (登録日: 2009-05-15, 公開日: 2009-07-07, 最終更新日: 2011-07-13)

主引用文献

Sainsbury, S.,Lane, L.A.,Ren, J.,Gilbert, R.J.,Saunders, N.J.,Robinson, C.V.,Stuart, D.I.,Owens, R.J.
The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators
Nucleic Acids Res., 37:4545-4558, 2009

PubMed Abstract: LysR-type transcriptional regulators (LTTRs) form the largest family of bacterial regulators acting as both auto-repressors and activators of target promoters, controlling operons involved in a wide variety of cellular processes. The LTTR, CrgA, from the human pathogen Neisseria meningitidis, is upregulated during bacterial-host cell contact. Here, we report the crystal structures of both regulatory domain and full-length CrgA, the first of a novel subclass of LTTRs that form octameric rings. Non-denaturing mass spectrometry analysis and analytical ultracentrifugation established that the octameric form of CrgA is the predominant species in solution in both the presence and absence of an oligonucleotide encompassing the CrgA-binding sequence. Furthermore, analysis of the isolated CrgA-DNA complex by mass spectrometry showed stabilization of a double octamer species upon DNA binding. Based on the observed structure and the mass spectrometry findings, a model is proposed in which a hexadecameric array of two CrgA oligomers binds to its DNA target site.

PubMed: 19474343
DOI: 10.1093/nar/gkp445

実験手法

X-RAY DIFFRACTION (2.3 Å)