3HDB

Crystal structure of AaHIV, A metalloproteinase from venom of Agkistrodon Acutus

3HDB の概要

• エントリーDOI: 10.2210/pdb3hdb/pdb
• 分子名称: AAHIV, KNL, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: aahiv, mdc-domain, disintegrin domain, cysteine-rich domain, metalloproteinase, disulfide bond, glycoprotein, hydrolase, metal-binding, metalloprotease, protease, secreted, toxin, zymogen
• 由来する生物種: Agkistrodon acutus (Hundred-pace snake); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49571.04

構造登録者

Zhu, Z.Q.,Niu, L.W.,Teng, M.K. (登録日: 2009-05-07, 公開日: 2009-08-11, 最終更新日: 2024-10-09)

主引用文献

Zhu, Z.,Gao, Y.,Zhu, Z.,Yu, Y.,Zhang, X.,Zang, J.,Teng, M.,Niu, L.
Structural basis of the autolysis of AaHIV suggests a novel target recognizing model for ADAM/reprolysin family proteins
Biochem.Biophys.Res.Commun., 386:159-164, 2009

PubMed Abstract: AaHIV, a P-III-type snake venom metalloproteinase (SVMP), consists of metalloproteinase/disintegrin/cysteine-rich (MDC) domains and is homologous to a disintegrin and metalloproteinase (ADAM) family proteins. Similar to brevilysin H6 and jararhagin, AaHIV can easily autolyse to release a stable protein named acucetin, which contains disintegrin-like and cysteine-rich domains. In this study, we determined the crystal structure of AaHIV and investigated the autolysis mechanism. Based on the structure of AaHIV and the results from docking experiments, we present a new model for target recognition in which two protein molecules form a functional unit, and the DC domain of one molecule is used for target recognition while the M-domain of the other is used for target proteolysis. Our results shed new light on the mechanism of target recognition and processing in ADAM/reprolysin family proteins.

PubMed: 19505434
DOI: 10.1016/j.bbrc.2009.06.004

実験手法

X-RAY DIFFRACTION (2.31 Å)