3HBE

Class IV chitinase structure from Picea abies at 1.55A

3HBE の概要

• エントリーDOI: 10.2210/pdb3hbe/pdb
• 関連するPDBエントリー: 3HBD 3HBH
• 分子名称: Class IV chitinase Chia4-Pa2, 2-METHOXYETHANOL, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: endochitinase, chitinase, class iv, family 19, conformational changes, chitin-binding, glycosidase, hydrolase
• 由来する生物種: Picea abies (Norway spruce)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21696.98

構造登録者

Ubhayasekera, W.,Mowbray, S.L. (登録日: 2009-05-04, 公開日: 2009-08-11, 最終更新日: 2023-09-06)

主引用文献

Ubhayasekera, W.,Rawat, R.,Ho, S.W.,Wiweger, M.,Von Arnold, S.,Chye, M.L.,Mowbray, S.L.
The first crystal structures of a family 19 class IV chitinase: the enzyme from Norway spruce.
Plant Mol.Biol., 71:277-289, 2009

PubMed Abstract: Chitinases help plants defend themselves against fungal attack, and play roles in other processes, including development. The catalytic modules of most plant chitinases belong to glycoside hydrolase family 19. We report here x-ray structures of such a module from a Norway spruce enzyme, the first for any family 19 class IV chitinase. The bi-lobed structure has a wide cleft lined by conserved residues; the most interesting for catalysis are Glu113, the proton donor, and Glu122, believed to be a general base that activate a catalytic water molecule. Comparisons to class I and II enzymes show that loop deletions in the class IV proteins make the catalytic cleft shorter and wider; from modeling studies, it is predicted that only three N-acetylglucosamine-binding subsites exist in class IV. Further, the structural comparisons suggest that the family 19 enzymes become more closed on substrate binding. Attempts to solve the structure of the complete protein including the associated chitin-binding module failed, however, modeling studies based on close relatives indicate that the binding module recognizes at most three N-acetylglucosamine units. The combined results suggest that the class IV enzymes are optimized for shorter substrates than the class I and II enzymes, or alternatively, that they are better suited for action on substrates where only small regions of chitin chain are accessible. Intact spruce chitinase is shown to possess antifungal activity, which requires the binding module; removing this module had no effect on measured chitinase activity.

PubMed: 19629717
DOI: 10.1007/s11103-009-9523-9

実験手法

X-RAY DIFFRACTION (1.55 Å)