3H8L

The first X-ray structure of a sulfide:quinone oxidoreductase: insights into sulfide oxidation mechanism

3H8L の概要

• エントリーDOI: 10.2210/pdb3h8l/pdb
• 関連するPDBエントリー: 3H8I
• 分子名称: NADH oxidase, FLAVIN-ADENINE DINUCLEOTIDE, trisulfane, ... (5 entities in total)
• 機能のキーワード: membrane protein, complete form, rossman-like fold, oxidoreductase
• 由来する生物種: Acidianus ambivalens (Desulfurolobus ambivalens)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92971.49

構造登録者

Brito, J.A.,Sousa, F.L.,Stelter, M.,Bandeiras, T.M.,Vonrhein, C.,Teixeira, M.,Pereira, M.M.,Archer, M. (登録日: 2009-04-29, 公開日: 2009-06-02, 最終更新日: 2024-10-16)

主引用文献

Brito, J.A.,Sousa, F.L.,Stelter, M.,Bandeiras, T.M.,Vonrhein, C.,Teixeira, M.,Pereira, M.M.,Archer, M.
Structural and functional insights into sulfide:quinone oxidoreductase.
Biochemistry, 48:5613-5622, 2009

PubMed Abstract: A sulfide:quinone oxidoreductase (SQR) was isolated from the membranes of the hyperthermoacidophilic archaeon Acidianus ambivalens, and its X-ray structure, the first reported for an SQR, was determined to 2.6 A resolution. This enzyme was functionally and structurally characterized and was shown to have two redox active sites: a covalently bound FAD and an adjacent pair of cysteine residues. Most interestingly, the X-ray structure revealed the presence of a chain of three sulfur atoms bridging those two cysteine residues. The possible implications of this observation in the catalytic mechanism for sulfide oxidation are discussed, and the role of SQR in the sulfur dependent bioenergetics of A. ambivalens, linked to oxygen reduction, is addressed.

PubMed: 19438211
DOI: 10.1021/bi9003827

実験手法

X-RAY DIFFRACTION (2.57 Å)