3H16

Crystal structure of a bacteria TIR domain, PdTIR from Paracoccus denitrificans

3H16 の概要

• エントリーDOI: 10.2210/pdb3h16/pdb
• 分子名称: TIR protein, SULFATE ION (3 entities in total)
• 機能のキーワード: bacteria tir domain, signaling protein
• 由来する生物種: Paracoccus denitrificans PD1222
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 68320.98

構造登録者

Chan, S.L.,Low, L.Y.,Santelli, E.,Pascual, J. (登録日: 2009-04-11, 公開日: 2009-06-16, 最終更新日: 2024-02-21)

主引用文献

Chan, S.L.,Low, L.Y.,Hsu, S.,Li, S.,Liu, T.,Santelli, E.,Le Negrate, G.,Reed, J.C.,Woods, V.L.,Pascual, J.
Molecular Mimicry in Innate Immunity: CRYSTAL STRUCTURE OF A BACTERIAL TIR DOMAIN.
J.Biol.Chem., 284:21386-21392, 2009

PubMed Abstract: Macrophages detect pathogen infection via the activation of their plasma membrane-bound Toll-like receptor proteins (TLRs). The heterotypic interaction between the Toll/interleukin-1 receptor (TIR) domains of TLRs and adaptor proteins, like Myeloid differentiation primary response gene 88 (MyD88), is the first intracellular step in the signaling pathway of the mammalian innate immune response. The hetero-oligomerization of the TIRs of the receptor and adaptor brings about the activation of the transcription factor NF-kappaB, which regulates the synthesis of pro-inflammatory cytokines. Here, we report the first crystal structure of a bacterial TIR domain solved at 2.5 A resolution. The three-dimensional fold of Paracoccus denitrificans TIR is identical to that observed for the TIR of human TLRs and MyD88 proteins. The structure shows a unique dimerization interface involving the DD-loop and EE-loop residues, whereas leaving the BB-loop highly exposed. Peptide amide hydrogen-deuterium exchange mass spectrometry also reveals that the same region is used for dimerization in solution and in the context of the full-length protein. These results, together with a functional interaction between P. denitrificans TIR and MyD88 visualized in a co-immunoprecipitation assay, further substantiate the model that bacterial TIR proteins adopt structural mimicry of the host active receptor TIR domains to interfere with the signaling of TLRs and their adaptors to decrease the inflammatory response.

PubMed: 19535337
DOI: 10.1074/jbc.C109.007591

実験手法

X-RAY DIFFRACTION (2.5 Å)