3H0N

Crystal structure of a duf1470 family protein (jann_2411) from jannaschia sp. ccs1 at 1.45 A resolution

Replaces:  2PW4

Summary for 3H0N

• Entry DOI: 10.2210/pdb3h0n/pdb
• Descriptor: uncharacterized protein DUF1470, ZINC ION, NICKEL (II) ION, ... (7 entities in total)
• Functional Keywords: treble clef zinc finger, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi-2, metal binding protein
• Biological source: Jannaschia sp. CCS1
• Total number of polymer chains: 1
• Total formula weight: 21375.54

Authors

Joint Center for Structural Genomics (JCSG) (deposition date: 2009-04-09, release date: 2009-04-21, Last modification date: 2023-02-01)

Primary citation

Bakolitsa, C.,Bateman, A.,Jin, K.K.,McMullan, D.,Krishna, S.S.,Miller, M.D.,Abdubek, P.,Acosta, C.,Astakhova, T.,Axelrod, H.L.,Burra, P.,Carlton, D.,Chiu, H.J.,Clayton, T.,Das, D.,Deller, M.C.,Duan, L.,Elias, Y.,Feuerhelm, J.,Grant, J.C.,Grzechnik, A.,Grzechnik, S.K.,Han, G.W.,Jaroszewski, L.,Klock, H.E.,Knuth, M.W.,Kozbial, P.,Kumar, A.,Marciano, D.,Morse, A.T.,Murphy, K.D.,Nigoghossian, E.,Okach, L.,Oommachen, S.,Paulsen, J.,Reyes, R.,Rife, C.L.,Sefcovic, N.,Tien, H.,Trame, C.B.,Trout, C.V.,van den Bedem, H.,Weekes, D.,White, A.,Xu, Q.,Hodgson, K.O.,Wooley, J.,Elsliger, M.A.,Deacon, A.M.,Godzik, A.,Lesley, S.,Wilson, I.A.
The structure of Jann_2411 (DUF1470) from Jannaschia sp. at 1.45  resolution reveals a new fold (the ABATE domain) and suggests its possible role as a transcription regulator.
Acta Crystallogr.,Sect.F, 66:1198-1204, 2010

PubMed Abstract: The crystal structure of Jann_2411 from Jannaschia sp. strain CCS1, a member of the Pfam PF07336 family classified as a domain of unknown function (DUF1470), was solved to a resolution of 1.45 Å by multiple-wavelength anomalous dispersion (MAD). This protein is the first structural representative of the DUF1470 Pfam family. Structural analysis revealed a two-domain organization, with the N-terminal domain presenting a new fold called the ABATE domain that may bind an as yet unknown ligand. The C-terminal domain forms a treble-clef zinc finger that is likely to be involved in DNA binding. Analysis of the Jann_2411 protein and the broader ABATE-domain family suggests a role as stress-induced transcriptional regulators.

PubMed: 20944211
DOI: 10.1107/S1744309109025196

Experimental method

X-RAY DIFFRACTION (1.45 Å)