3GWH

Crystallographic Ab Initio protein solution far below atomic resolution

Summary for 3GWH

• Entry DOI: 10.2210/pdb3gwh/pdb
• Descriptor: Transcriptional antiterminator (BglG family), PHOSPHATE ION (3 entities in total)
• Functional Keywords: extended helix bundle, ab initio, structure solution, arcimboldo, phaser, shelxe, transcription
• Biological source: Bacillus subtilis
• Total number of polymer chains: 2
• Total formula weight: 26498.90

Authors

Rodriguez, D.D.,Grosse, C.,Himmel, S.,Gonzalez, C.,Becker, S.,Sheldrick, G.M.,Uson, I. (deposition date: 2009-04-01, release date: 2010-04-07, Last modification date: 2024-03-20)

Primary citation

Rodriguez, D.D.,Grosse, C.,Himmel, S.,Gonzalez, C.,de Ilarduya, I.M.,Becker, S.,Sheldrick, G.M.,Uson, I.
Crystallographic ab initio protein structure solution below atomic resolution
Nat.Methods, 6:651-653, 2009

PubMed Abstract: Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 A) unless heavy atoms are present. We describe a general ab initio phasing method for 2 A data, based on combination of localizing model fragments such as small á-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 A.

PubMed: 19684596
DOI: 10.1038/nmeth.1365

Experimental method

X-RAY DIFFRACTION (1.95 Å)