3GT4

Structure of proteinase K with the magic triangle I3C

3GT4 の概要

• エントリーDOI: 10.2210/pdb3gt4/pdb
• 関連するPDBエントリー: 3E3D 3E3S 3E3T 3GT3
• 分子名称: proteinase K, 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: phasing tool, 5-amino-2, 4, 6-triiodoisophthalic acid, i3c, magic triangle, 6-tribromoisophthalic acid, b3c, mad triangle, disulfide bond, hydrolase, metal-binding, protease, serine protease, zymogen
• 由来する生物種: Engyodontium album
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30731.36

構造登録者

Beck, T.,Gruene, T.,Sheldrick, G.M. (登録日: 2009-03-27, 公開日: 2009-04-14, 最終更新日: 2024-11-13)

主引用文献

Beck, T.,Gruene, T.,Sheldrick, G.M.
The magic triangle goes MAD: experimental phasing with a bromine derivative
Acta Crystallogr.,Sect.D, 66:374-380, 2010

PubMed Abstract: Experimental phasing is an essential technique for the solution of macromolecular structures. Since many heavy-atom ion soaks suffer from nonspecific binding, a novel class of compounds has been developed that combines heavy atoms with functional groups for binding to proteins. The phasing tool 5-amino-2,4,6-tribromoisophthalic acid (B3C) contains three functional groups (two carboxylate groups and one amino group) that interact with proteins via hydrogen bonds. Three Br atoms suitable for anomalous dispersion phasing are arranged in an equilateral triangle and are thus readily identified in the heavy-atom substructure. B3C was incorporated into proteinase K and a multiwavelength anomalous dispersion (MAD) experiment at the Br K edge was successfully carried out. Radiation damage to the bromine-carbon bond was investigated. A comparison with the phasing tool I3C that contains three I atoms for single-wavelength anomalous dispersion (SAD) phasing was also carried out.

PubMed: 20382990
DOI: 10.1107/S0907444909051609

実験手法

X-RAY DIFFRACTION (1.76 Å)