3GQC

Structure of human Rev1-DNA-dNTP ternary complex

3GQC の概要

• エントリーDOI: 10.2210/pdb3gqc/pdb
• 分子名称: DNA repair protein REV1, 5'-D(*AP*TP*CP*CP*TP*CP*CP*CP*CP*TP*AP*(DOC))-3', 5'-D(*TP*AP*AP*GP*GP*TP*AP*GP*GP*GP*GP*AP*GP*GP*AP*T)-3', ... (6 entities in total)
• 機能のキーワード: protein-dna complex, dna damage, dna repair, dna synthesis, dna-binding, magnesium, metal-binding, nucleotidyltransferase, nucleus, transferase, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus (Probable): Q9UBZ9
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 260167.25

構造登録者

Swan, M.K.,Aggarwal, A.K. (登録日: 2009-03-24, 公開日: 2009-05-19, 最終更新日: 2023-09-06)

主引用文献

Swan, M.K.,Johnson, R.E.,Prakash, L.,Prakash, S.,Aggarwal, A.K.
Structure of the human Rev1-DNA-dNTP ternary complex.
J.Mol.Biol., 390:699-709, 2009

PubMed Abstract: Y-family DNA polymerases have proven to be remarkably diverse in their functions and in strategies for replicating through DNA lesions. The structure of yeast Rev1 ternary complex has revealed the most radical replication strategy, where the polymerase itself dictates the identity of the incoming nucleotide, as well as the identity of the templating base. We show here that many of the key elements of this highly unusual strategy are conserved between yeast and human Rev1, including the eviction of template G from the DNA helix and the pairing of incoming deoxycytidine 5'-triphosphate with a surrogate arginine residue. We also show that the catalytic core of human Rev1 is uniquely augmented by two large inserts, I1 and I2, wherein I1 extends >20 A away from the active site and may serve as a platform for protein-protein interactions specific for Rev1's role in translesion DNA synthesis in human cells, and I2 acts as a "flap" on the hydrophobic pocket accommodating template G. We suggest that these novel structural features are important for providing human Rev1 greater latitude in promoting efficient and error-free translesion DNA synthesis through the diverse array of bulky and potentially carcinogenic N(2)-deoxyguanosine DNA adducts in human cells.

PubMed: 19464298
DOI: 10.1016/j.jmb.2009.05.026

実験手法

X-RAY DIFFRACTION (2.5 Å)