3GP5

Crystal structure of phosphoglyceromutase from Burkholderia pseudomallei with 3-phosphoglyceric acid and vanadate

3GP5 の概要

• エントリーDOI: 10.2210/pdb3gp5/pdb
• 関連するPDBエントリー: 3EZN 3FDZ 3GP3 3GW8
• 分子名称: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase, 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE, VANADATE ION, ... (6 entities in total)
• 機能のキーワード: phosphoglyceromutase, decode, uwppg. sbri, niaid, ssgcid, glycolysis, isomerase, structural genomics, seattle structural genomics center for infectious disease
• 由来する生物種: Burkholderia pseudomallei (Pseudomonas pseudomallei)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59174.46

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2009-03-20, 公開日: 2009-03-31, 最終更新日: 2024-02-21)

主引用文献

Davies, D.R.,Staker, B.L.,Abendroth, J.A.,Edwards, T.E.,Hartley, R.,Leonard, J.,Kim, H.,Rychel, A.L.,Hewitt, S.N.,Myler, P.J.,Stewart, L.J.
An ensemble of structures of Burkholderia pseudomallei 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase.
Acta Crystallogr.,Sect.F, 67:1044-1050, 2011

PubMed Abstract: Burkholderia pseudomallei is a soil-dwelling bacterium endemic to Southeast Asia and Northern Australia. Burkholderia is responsible for melioidosis, a serious infection of the skin. The enzyme 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (PGAM) catalyzes the interconversion of 3-phosphoglycerate and 2-phosphoglycerate, a key step in the glycolytic pathway. As such it is an extensively studied enzyme and X-ray crystal structures of PGAM enzymes from multiple species have been elucidated. Vanadate is a phosphate mimic that is a powerful tool for studying enzymatic mechanisms in phosphoryl-transfer enzymes such as phosphoglycerate mutase. However, to date no X-ray crystal structures of phosphoglycerate mutase have been solved with vanadate acting as a substrate mimic. Here, two vanadate complexes together with an ensemble of substrate and fragment-bound structures that provide a comprehensive picture of the function of the Burkholderia enzyme are reported.

PubMed: 21904048
DOI: 10.1107/S1744309111030405

実験手法

X-RAY DIFFRACTION (2.25 Å)