3GL4

X-ray structure of photobleached killerred

3GL4 の概要

• エントリーDOI: 10.2210/pdb3gl4/pdb
• 関連するPDBエントリー: 3GB3
• 分子名称: KillerRed (2 entities in total)
• 機能のキーワード: fluorescent protein, genetically encoded photosensitizer, killerred, phototoxicity
• 由来する生物種: Anthomedusae sp. DC-2005
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52827.43

構造登録者

Pletnev, S.,Pletneva, N.V.,Pletnev, V.Z. (登録日: 2009-03-11, 公開日: 2009-09-08, 最終更新日: 2026-03-18)

主引用文献

Pletnev, S.,Gurskaya, N.G.,Pletneva, N.V.,Lukyanov, K.A.,Chudakov, D.M.,Martynov, V.I.,Popov, V.O.,Kovalchuk, M.V.,Wlodawer, A.,Dauter, Z.,Pletnev, V.
Structural basis for phototoxicity of the genetically encoded photosensitizer KillerRed.
J.Biol.Chem., 284:32028-32039, 2009

PubMed Abstract: KillerRed is the only known fluorescent protein that demonstrates notable phototoxicity, exceeding that of the other green and red fluorescent proteins by at least 1,000-fold. KillerRed could serve as an instrument to inactivate target proteins or to kill cell populations in photodynamic therapy. However, the nature of KillerRed phototoxicity has remained unclear, impeding the development of more phototoxic variants. Here we present the results of a high resolution crystallographic study of KillerRed in the active fluorescent and in the photobleached non-fluorescent states. A unique and striking feature of the structure is a water-filled channel reaching the chromophore area from the end cap of the beta-barrel that is probably one of the key structural features responsible for phototoxicity. A study of the structure-function relationship of KillerRed, supported by structure-based, site-directed mutagenesis, has also revealed the key residues most likely responsible for the phototoxic effect. In particular, Glu(68) and Ser(119), located adjacent to the chromophore, have been assigned as the primary trigger of the reaction chain.

PubMed: 19737938
DOI: 10.1074/jbc.M109.054973

実験手法

X-RAY DIFFRACTION (2.15 Å)