3GJ2

Photoactivated state of PA-GFP

3GJ2 の概要

• エントリーDOI: 10.2210/pdb3gj2/pdb
• 関連するPDBエントリー: 3GJ1
• 分子名称: Green fluorescent protein, CHLORIDE ION (3 entities in total)
• 機能のキーワード: beta barrel, chromophore, luminescence, photoprotein, luminescent protein
• 由来する生物種: Aequorea victoria (Jellyfish)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 104006.97

構造登録者

Henderson, J.N.,Gepshtein, R.,Heenan, J.R.,Kallio, K.,Huppert, D.,Remington, S.J. (登録日: 2009-03-07, 公開日: 2009-03-24, 最終更新日: 2026-03-18)

主引用文献

Henderson, J.N.,Gepshtein, R.,Heenan, J.R.,Kallio, K.,Huppert, D.,Remington, S.J.
Structure and mechanism of the photoactivatable green fluorescent protein.
J.Am.Chem.Soc., 131:4176-4177, 2009

PubMed Abstract: Crystal structures of the photoactivatable green fluorescent protein T203H variant (PA-GFP) have been solved in the native and photoactivated states, which under 488 nm illumination are dark and brightly fluorescent, respectively. We demonstrate that photoactivation of PA-GFP is the result of a UV-induced decarboxylation of the Glu222 side chain that shifts the chromophore equilibrium to the anionic form. Coupled with the T203H mutation, which stabilizes the native PA-GFP neutral chromophore, Glu222 decarboxylation yields a 100-fold contrast enhancement relative to wild-type GFP (WT). Additionally, the structures provide insights into the spectroscopic differences between WT and PA-GFP steady-state fluorescence maxima and excited-state proton transfer dynamics.

PubMed: 19278226
DOI: 10.1021/ja808851n

実験手法

X-RAY DIFFRACTION (1.9 Å)