3GHB

Crystal structure of anti-HIV-1 Fab 447-52D in complex with V3 peptide W2RW020

3GHB の概要

• エントリーDOI: 10.2210/pdb3ghb/pdb
• 関連するPDBエントリー: 1Q1J 3C2A 3GHE
• 分子名称: Fab 447-52D, light chain, Fab 447-52D, heavy chain, Envelope glycoprotein, ... (5 entities in total)
• 機能のキーワード: hiv, v3 loop, antibody-antigen interactions, envelope protein, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 98023.44

構造登録者

Kong, X.P.,Burke, V.J. (登録日: 2009-03-03, 公開日: 2009-12-01, 最終更新日: 2024-11-20)

主引用文献

Burke, V.,Williams, C.,Sukumaran, M.,Kim, S.S.,Li, H.,Wang, X.H.,Gorny, M.K.,Zolla-Pazner, S.,Kong, X.P.
Structural basis of the cross-reactivity of genetically related human anti-HIV-1 mAbs: implications for design of V3-based immunogens
Structure, 17:1538-1546, 2009

PubMed Abstract: Human monoclonal antibodies 447-52D and 537-10D, both coded by the VH3 gene and specific for the third variable region (V3) of the HIV-1 gp120, were found to share antigen-binding structural elements including an elongated CDR H3 forming main-chain interactions with the N terminus of the V3 crown. However, water-mediated hydrogen bonds and a unique cation-pi sandwich stacking allow 447-52D to be broadly reactive with V3 containing both the GPGR and GPGQ crown motifs, while the deeper binding pocket and a buried Glu in the binding site of 537-10D limit its reactivity to only V3 containing the GPGR motif. Our results suggest that the design of immunogens for anti-V3 antibodies should avoid the Arg at the V3 crown, as GPGR-containing epitopes appear to select for B cells making antibodies of narrower specificity than V3 that carry Gln at this position.

PubMed: 19913488
DOI: 10.1016/j.str.2009.09.012

実験手法

X-RAY DIFFRACTION (2.25 Å)