3GF2

Crystal structure of the hypothetical regulator ST1710 complexed with sodium salicylate

3GF2 の概要

• エントリーDOI: 10.2210/pdb3gf2/pdb
• 関連するPDBエントリー: 2eb7 2yr2 3GFI 3GFJ 3GFL 3GFM 3gez
• 分子名称: 146aa long hypothetical transcriptional regulator, 2-HYDROXYBENZOIC ACID (3 entities in total)
• 機能のキーワード: transcription regulator, st1710, marr, dna-binding, transcription, transcription regulation, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi
• 由来する生物種: Sulfolobus tokodaii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17017.68

構造登録者

Kumarevel, T.,Tanaka, T.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2009-02-26, 公開日: 2009-08-25, 最終更新日: 2023-11-01)

主引用文献

Kumarevel, T.,Tanaka, T.,Umehara, T.,Yokoyama, S.
ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators.
Nucleic Acids Res., 37:4723-4735, 2009

PubMed Abstract: ST1710, a member of the multiple antibiotic resistance regulator (MarR) family of regulatory proteins in bacteria and archaea, plays important roles in development of antibiotic resistance, a global health problem. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with salicylate, a well-known inhibitor of MarR proteins and the ST1710 complex with its promoter DNA, refined to 1.8 and 2.10 A resolutions, respectively. The ST1710-DNA complex shares the topology of apo-ST1710 and MarR proteins, with each subunit containing a winged helix-turn-helix (wHtH) DNA binding motif. Significantly large conformational changes occurred upon DNA binding and in each of the dimeric monomers in the asymmetric unit of the ST1710-DNA complex. Conserved wHtH loop residues interacting with the bound DNA and mutagenic analysis indicated that R89, R90 and K91 were important for DNA recognition. Significantly, the bound DNA exhibited a new binding mechanism.

PubMed: 19509310
DOI: 10.1093/nar/gkp496

実験手法

X-RAY DIFFRACTION (1.8 Å)