3GCG

crystal structure of MAP and CDC42 complex

3GCG の概要

• エントリーDOI: 10.2210/pdb3gcg/pdb
• 分子名称: Cell division control protein 42 homolog, L0028 (Mitochondria associated protein) (3 entities in total)
• 機能のキーワード: map, cdc42, complex, alternative splicing, cell membrane, gtp-binding, lipoprotein, membrane, methylation, nucleotide-binding, prenylation cdc42, signaling protein-transcription complex, signaling protein/transcription
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential): P60953
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39778.07

構造登録者

Chai, J.,Huang, Z.,Feng, Y.,Wu, X. (登録日: 2009-02-22, 公開日: 2009-07-21, 最終更新日: 2024-03-20)

主引用文献

Huang, Z.,Sutton, S.E.,Wallenfang, A.J.,Orchard, R.C.,Wu, X.,Feng, Y.,Chai, J.,Alto, N.M.
Structural insights into host GTPase isoform selection by a family of bacterial GEF mimics
Nat.Struct.Mol.Biol., 16:853-860, 2009

PubMed Abstract: The Escherichia coli type III effector Map belongs to a large family of bacterial virulence factors that activate host Rho GTPase signaling pathways through an unknown molecular mechanism. Here we report direct evidence that Map functions as a potent and selective guanine-nucleotide exchange factor (GEF) for Cdc42. The 2.3-A structure of the Map-Cdc42 complex revealed that Map mimics the GEF strategy of the mammalian Dbl family but has a three-dimensional architecture that is nearly identical to the bacterial GEF Salmonella spp. SopE. A comparative analysis between human and bacterial GEFs revealed a previously uncharacterized pairing mechanism between Map and the variable beta2-3 interswitch region of Cdc42. We propose a GTPase selection model that is experimentally validated by the preferential activation Rac1 and RhoA by the Shigella spp. effectors IpgB1 and IpgB2, respectively. These results significantly expand the repertoire of bacterial GEF mimics and unify a GEF selection mechanism for host GTPase substrates.

PubMed: 19620963
DOI: 10.1038/nsmb.1647

実験手法

X-RAY DIFFRACTION (2.3 Å)