3GCC

SOLUTION STRUCTURE OF THE GCC-BOX BINDING DOMAIN, NMR, 46 STRUCTURES

Summary for 3GCC

• Entry DOI: 10.2210/pdb3gcc/pdb
• Descriptor: ATERF1 (1 entity in total)
• Functional Keywords: transcription factor, ethlene inducible
• Biological source: Arabidopsis thaliana (thale cress)
• Cellular location: Nucleus (Probable): O80337
• Total number of polymer chains: 1
• Total formula weight: 7891.97

Authors

Allen, M.D.,Yamasaki, K.,Ohme-Takagi, M.,Tateno, M.,Suzuki, M. (deposition date: 1998-03-13, release date: 1999-03-23, Last modification date: 2024-05-22)

Primary citation

Allen, M.D.,Yamasaki, K.,Ohme-Takagi, M.,Tateno, M.,Suzuki, M.
A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA.
EMBO J., 17:5484-5496, 1998

PubMed Abstract: The 3D solution structure of the GCC-box binding domain of a protein from Arabidopsis thaliana in complex with its target DNA fragment has been determined by heteronuclear multidimensional NMR in combination with simulated annealing and restrained molecular dynamic calculation. The domain consists of a three-stranded anti-parallel beta-sheet and an alpha-helix packed approximately parallel to the beta-sheet. Arginine and tryptophan residues in the beta-sheet are identified to contact eight of the nine consecutive base pairs in the major groove, and at the same time bind to the sugar phosphate backbones. The target DNA bends slightly at the central CG step, thereby allowing the DNA to follow the curvature of the beta-sheet.

PubMed: 9736626
DOI: 10.1093/emboj/17.18.5484

Experimental method

SOLUTION NMR