3GBD

Crystal structure of the isomaltulose synthase SmuA from Protaminobacter rubrum

3GBD の概要

• エントリーDOI: 10.2210/pdb3gbd/pdb
• 関連するPDBエントリー: 3GBE
• 分子名称: Sucrose isomerase SmuA from Protaminobacter rubrum, 1,2-ETHANEDIOL, CITRATE ANION, ... (4 entities in total)
• 機能のキーワード: sucrose isomerase, glycoside hydrolase, protaminobacter rubrum, isomerase
• 由来する生物種: Protaminobacter rubrum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 66374.00

構造登録者

Ravaud, S.,Robert, X.,Haser, R.,Aghajari, N. (登録日: 2009-02-19, 公開日: 2009-05-26, 最終更新日: 2023-11-01)

主引用文献

Ravaud, S.,Robert, X.,Watzlawick, H.,Haser, R.,Mattes, R.,Aghajari, N.
Structural determinants of product specificity of sucrose isomerases
Febs Lett., 583:1964-1968, 2009

PubMed Abstract: The healthy sweetener isomaltulose is industrially produced from the conversion of sucrose by the sucrose isomerase SmuA from Protaminobacter rubrum. Crystal structures of SmuA in native and deoxynojirimycin complexed forms completed with modeling studies unravel the characteristics of the isomaltulose synthases catalytic pocket and their substrate binding mode. Comparison with the trehalulose synthase MutB highlights the role of Arg(298) and Arg(306) active site residues and surface charges in controlling product specificity of sucrose isomerases (isomaltulose versus trehalulose). The results provide a rationale for the specific design of optimized enzymes.

PubMed: 19427862
DOI: 10.1016/j.febslet.2009.05.002

実験手法

X-RAY DIFFRACTION (1.95 Å)