3G9A

Green fluorescent protein bound to minimizer nanobody

3G9A の概要

• エントリーDOI: 10.2210/pdb3g9a/pdb
• 関連するPDBエントリー: 3G8J
• 分子名称: Green fluorescent protein, Minimizer (3 entities in total)
• 機能のキーワード: antibody complex, chromophore, luminescence, photoprotein, fluorescent protein-immune system complex, nanobody, fluorescent protein/immune system
• 由来する生物種: Aequorea victoria (Jellyfish); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41782.68

構造登録者

Kirchhofer, A.,Helma, J.,Schmidthals, K.,Frauer, C.,Cui, S.,Karcher, A.,Pellis, M.,Muyldermans, S.,Delucci, C.C.,Cardoso, M.C.,Leonhardt, H.,Hopfner, K.-P.,Rothbauer, U. (登録日: 2009-02-13, 公開日: 2009-12-08, 最終更新日: 2024-10-30)

主引用文献

Kirchhofer, A.,Helma, J.,Schmidthals, K.,Frauer, C.,Cui, S.,Karcher, A.,Pellis, M.,Muyldermans, S.,Deulcci, C.C.,Cardoso, M.C.,Leonhardt, H.,Hopfner, K.-P.,Rothbauer, U.
Modulation of protein properties in living cells using nanobodies
Nat.Struct.Mol.Biol., 17:133-138, 2010

PubMed Abstract: Protein conformation is critically linked to function and often controlled by interactions with regulatory factors. Here we report the selection of camelid-derived single-domain antibodies (nanobodies) that modulate the conformation and spectral properties of the green fluorescent protein (GFP). One nanobody could reversibly reduce GFP fluorescence by a factor of 5, whereas its displacement by a second nanobody caused an increase by a factor of 10. Structural analysis of GFP-nanobody complexes revealed that the two nanobodies induce subtle opposing changes in the chromophore environment, leading to altered absorption properties. Unlike conventional antibodies, the small, stable nanobodies are functional in living cells. Nanobody-induced changes were detected by ratio imaging and used to monitor protein expression and subcellular localization as well as translocation events such as the tamoxifen-induced nuclear localization of estrogen receptor. This work demonstrates that protein conformations can be manipulated and studied with nanobodies in living cells.

PubMed: 20010839
DOI: 10.1038/nsmb.1727

実験手法

X-RAY DIFFRACTION (1.614 Å)