3G6U

GR DNA-binding domain:FKBP5 16bp complex-49

3G6U の概要

• エントリーDOI: 10.2210/pdb3g6u/pdb
• 関連するPDBエントリー: 3FYL 3G6P 3G6Q 3G6R 3G6T 3G8X 3G97 3G99
• 分子名称: Glucocorticoid receptor, DNA (5'-D(*TP*AP*GP*AP*AP*CP*AP*GP*GP*GP*TP*GP*TP*TP*CP*T)-3'), DNA (5'-D(*AP*AP*GP*AP*AP*CP*AP*CP*CP*CP*TP*GP*TP*TP*CP*T)-3'), ... (5 entities in total)
• 機能のキーワード: glucocorticoid, dna-binding, allostery, lever arm, transcription, hormone, alternative initiation, chromatin regulator, cytoplasm, lipid-binding, metal-binding, nucleus, phosphoprotein, polymorphism, receptor, steroid-binding, transcription regulation, ubl conjugation, zinc, zinc-finger, transcription-dna complex, transcription/dna
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats)
• 細胞内の位置: Isoform A: Cytoplasm : P06536
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 29982.55

構造登録者

Pufall, M.A.,Yamamoto, K.R.,Meijsing, S.H. (登録日: 2009-02-08, 公開日: 2009-04-21, 最終更新日: 2023-09-06)

主引用文献

Meijsing, S.H.,Pufall, M.A.,So, A.Y.,Bates, D.L.,Chen, L.,Yamamoto, K.R.
DNA binding site sequence directs glucocorticoid receptor structure and activity.
Science, 324:407-410, 2009

PubMed Abstract: Genes are not simply turned on or off, but instead their expression is fine-tuned to meet the needs of a cell. How genes are modulated so precisely is not well understood. The glucocorticoid receptor (GR) regulates target genes by associating with specific DNA binding sites, the sequences of which differ between genes. Traditionally, these binding sites have been viewed only as docking sites. Using structural, biochemical, and cell-based assays, we show that GR binding sequences, differing by as little as a single base pair, differentially affect GR conformation and regulatory activity. We therefore propose that DNA is a sequence-specific allosteric ligand of GR that tailors the activity of the receptor toward specific target genes.

PubMed: 19372434
DOI: 10.1126/science.1164265

実験手法

X-RAY DIFFRACTION (1.9 Å)