3G4M

Crystal structure of guanine riboswitch bound to 2-aminopurine

3G4M の概要

• エントリーDOI: 10.2210/pdb3g4m/pdb
• 関連するPDBエントリー: 1U8D 3GAO 3GER 3GES
• 分子名称: Guanine riboswitch, 9H-purin-2-amine, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: riboswitch, mrna, 2-aminopurine, guanine, three-way junction, rna-ligand complex, double helix, base-triple., rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23473.23

構造登録者

Gilbert, S.D.,Batey, R.T. (登録日: 2009-02-04, 公開日: 2009-06-23, 最終更新日: 2023-09-06)

主引用文献

Gilbert, S.D.,Reyes, F.E.,Edwards, A.L.,Batey, R.T.
Adaptive ligand binding by the purine riboswitch in the recognition of Guanine and adenine analogs.
Structure, 17:857-868, 2009

PubMed Abstract: Purine riboswitches discriminate between guanine and adenine by at least 10,000-fold based on the identity of a single pyrimidine (Y74) that forms a Watson-Crick base pair with the ligand. To understand how this high degree of specificity for closely related compounds is achieved through simple pairing, we investigated their interaction with purine analogs with varying functional groups at the 2- and 6-positions that have the potential to alter interactions with Y74. Using a combination of crystallographic and calorimetric approaches, we find that binding these purines is often facilitated by either small structural changes in the RNA or tautomeric changes in the ligand. This work also reveals that, along with base pairing, conformational restriction of Y74 significantly contributes to nucleobase selectivity. These results reveal that compounds that exploit the inherent local flexibility within riboswitch binding pockets can alter their ligand specificity.

PubMed: 19523903
DOI: 10.1016/j.str.2009.04.009

実験手法

X-RAY DIFFRACTION (2.4 Å)