3FZN

Intermediate analogue in benzoylformate decarboxylase

Summary for 3FZN

Entry DOI	10.2210/pdb3fzn/pdb
Descriptor	Benzoylformate decarboxylase, 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(S)-hydroxy[(R)-hydroxy(methoxy)phosphoryl]phenylmethyl}-5-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium, MAGNESIUM ION, ... (7 entities in total)
Functional Keywords	benzoylformate decarboxylase, thiamin diphosphate, intermediate analogue, aromatic hydrocarbons catabolism, calcium, decarboxylase, lyase, magnesium, mandelate pathway, metal-binding, thiamine pyrophosphate
Biological source	Pseudomonas putida
Total number of polymer chains	4
Total formula weight	232518.81
Authors	Bruning, M.,Berheide, M.,Meyer, D.,Golbik, R.,Bartunik, H.,Liese, A.,Tittmann, K. (deposition date: 2009-01-26, release date: 2009-05-05, Last modification date: 2023-11-01)
Primary citation	Bruning, M.,Berheide, M.,Meyer, D.,Golbik, R.,Bartunik, H.,Liese, A.,Tittmann, K. Structural and kinetic studies on native intermediates and an intermediate analogue in benzoylformate decarboxylase reveal a least motion mechanism with an unprecedented short-lived predecarboxylation intermediate. Biochemistry, 48:3258-3268, 2009 Cited by PubMed: 19182954 DOI: 10.1021/bi801957d PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.62 Å)

Structure validation