3FW6

Crystal structure of CelM2, a bifunctional glucanase-xylanase protein from a metagenome library

3FW6 の概要

• エントリーDOI: 10.2210/pdb3fw6/pdb
• 分子名称: Cellulase, ZINC ION (3 entities in total)
• 機能のキーワード: celm2, glucanase-xyanase, glucanase, xylanase, bifunctional enzyme, hydrolase
• 由来する生物種: uncultured bacterium
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58367.20

構造登録者

Hwang, K.Y.,Nam, K.H. (登録日: 2009-01-17, 公開日: 2009-03-03, 最終更新日: 2023-11-01)

主引用文献

Nam, K.H.,Kim, S.-J.,Hwang, K.Y.
Crystal structure of CelM2, a bifunctional glucanase-xylanase protein from a metagenome library
Biochem.Biophys.Res.Commun., 383:183-186, 2009

PubMed Abstract: Degradation of polysaccharides by cellulases and xylanases plays an important role in the carbon cycle, but only occurs in plant cell walls, a few bacteria and some animals. This process is also critical in processes such as biomass degradation and fuel production in the conversion cycles of cellulosic biomass. The enzyme CelM2 is bifunctional, because it is able to effectively hydrolyze barley glucan and xylan. Here, we show the crystal structure of the bifunctional enzyme CelM2, isolated from a metagenome library, and describe the sequence information and structure of its two domains. We believe that CelM2 is attractive as an industrial enzyme and that the structural results presented herein provide insights that are relevant to the genetic engineering of multifunctional enzymes.

PubMed: 19345197
DOI: 10.1016/j.bbrc.2009.03.149

実験手法

X-RAY DIFFRACTION (2.3 Å)