3FO4
Crystal structure of guanine riboswitch C74U mutant bound to 6-chloroguanine
Summary for 3FO4
| Entry DOI | 10.2210/pdb3fo4/pdb |
| Related | 1U8D 3FO6 |
| Descriptor | Guanine riboswitch C74U mutant, 6-chloroguanine, ACETATE ION, ... (5 entities in total) |
| Functional Keywords | mrna, riboswitch, 6-chloroguanine, adenine, rna-ligand complex, double helix, three-way junction, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 23998.98 |
| Authors | Gilbert, S.D.,Batey, R.T. (deposition date: 2008-12-27, release date: 2009-06-23, Last modification date: 2023-09-06) |
| Primary citation | Gilbert, S.D.,Reyes, F.E.,Edwards, A.L.,Batey, R.T. Adaptive ligand binding by the purine riboswitch in the recognition of Guanine and adenine analogs. Structure, 17:857-868, 2009 Cited by PubMed Abstract: Purine riboswitches discriminate between guanine and adenine by at least 10,000-fold based on the identity of a single pyrimidine (Y74) that forms a Watson-Crick base pair with the ligand. To understand how this high degree of specificity for closely related compounds is achieved through simple pairing, we investigated their interaction with purine analogs with varying functional groups at the 2- and 6-positions that have the potential to alter interactions with Y74. Using a combination of crystallographic and calorimetric approaches, we find that binding these purines is often facilitated by either small structural changes in the RNA or tautomeric changes in the ligand. This work also reveals that, along with base pairing, conformational restriction of Y74 significantly contributes to nucleobase selectivity. These results reveal that compounds that exploit the inherent local flexibility within riboswitch binding pockets can alter their ligand specificity. PubMed: 19523903DOI: 10.1016/j.str.2009.04.009 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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