3FNQ
Crystal structure of schistosoma purine nucleoside phosphorylase in complex with hypoxanthine
Summary for 3FNQ
| Entry DOI | 10.2210/pdb3fnq/pdb |
| Related | 1TCV 1TD1 3FAZ 3FB1 |
| Descriptor | Purine-nucleoside phosphorylase, HYPOXANTHINE, SULFATE ION, ... (5 entities in total) |
| Functional Keywords | purine nucleoside phosphorylase, glycosyltransferase, transferase |
| Biological source | Schistosoma mansoni (Blood fluke) |
| Total number of polymer chains | 3 |
| Total formula weight | 94522.68 |
| Authors | Castilho, M.S.,Pereira, H.M.,Garratt, R.C.,Oliva, G. (deposition date: 2008-12-26, release date: 2009-02-24, Last modification date: 2023-11-01) |
| Primary citation | Pereira, H.M.,Rezende, M.M.,Castilho, M.S.,Oliva, G.,Garratt, R.C. Adenosine binding to low-molecular-weight purine nucleoside phosphorylase: the structural basis for recognition based on its complex with the enzyme from Schistosoma mansoni. Acta Crystallogr.,Sect.D, 66:73-79, 2010 Cited by PubMed Abstract: Schistosomes are unable to synthesize purines de novo and depend exclusively on the salvage pathway for their purine requirements. It has been suggested that blockage of this pathway could lead to parasite death. The enzyme purine nucleoside phosphorylase (PNP) is one of its key components and molecules designed to inhibit the low-molecular-weight (LMW) PNPs, which include both the human and schistosome enzymes, are typically analogues of the natural substrates inosine and guanosine. Here, it is shown that adenosine both binds to Schistosoma mansoni PNP and behaves as a weak micromolar inhibitor of inosine phosphorolysis. Furthermore, the first crystal structures of complexes of an LMW PNP with adenosine and adenine are reported, together with those with inosine and hypoxanthine. These are used to propose a structural explanation for the selective binding of adenosine to some LMW PNPs but not to others. The results indicate that transition-state analogues based on adenosine or other 6-amino nucleosides should not be discounted as potential starting points for alternative inhibitors. PubMed: 20057051DOI: 10.1107/S0907444909045715 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.85 Å) |
Structure validation
Download full validation report
