3FL6

Influence of the incorporation of a cyclohexenyl nucleic acid (CeNA) residue onto the sequence d(GCGTGCG)/d(CGCACGC)

3FL6 の概要

• エントリーDOI: 10.2210/pdb3fl6/pdb
• 関連するPDBエントリー: 2h0n 2p8d
• 分子名称: 5'-D(*GP*CP*GP*(XTR)P*GP*CP*G)-3', 5'-D(*CP*GP*CP*AP*CP*GP*C)-3', COBALT HEXAMMINE(III), ... (4 entities in total)
• 機能のキーワード: double helix, cena, sugar modification, right-handed, dna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 9623.51

構造登録者

Robeyns, K.,Herdewijn, P.,Van Meervelt, L. (登録日: 2008-12-18, 公開日: 2009-12-29, 最終更新日: 2024-02-21)

主引用文献

Robeyns, K.,Herdewijn, P.,Van Meervelt, L.
Direct observation of two cyclohexenyl (CeNA) ring conformations in duplex DNA.
Artif DNA PNA XNA, 1:2-8, 2010

PubMed Abstract: Cyclohexene Nucleic Acids (CeNA), in which the 2'-deoxyribofuranose ring of the DNA building blocks is substituted by a cyclohexenyl ring, were designed as potential mimics of natural nucleic acids for antisense and, later, for siRNA applications. CeNA units, in contrast to HNA (hexitol nucleic acid) building blocks, show more flexibility at the level of the C2'-C3' bond due to the possibility of the cyclohexenyl moiety to adopt different conformations. In order to analyze the influence of CeNA residues onto the helix conformation and hydration of natural nucleic acid structures and to verify the cyclohexenyl ring conformation, a cyclohexenyl-thymine building block was incorporated into the non-self-complementary sequence d(GCG(xT)GCG)/d(CGCACGC) with (xT) a cyclohexene residue. The crystal structure of this sequence has been determined to a resolution of 1.17 Å and contains two duplexes in the asymmetric unit. The global helices belong to the B-type family and the conformations of the cyclohexenyl rings in both duplexes are different. The cyclohexene ring adopts as well the (2)H(3)-conformation (similar to C2'-endo) as the (3)H(2)-conformation (similar to C3'-endo). The crystal packing is stabilized by cobalt hexamine residues and triplet formation.

PubMed: 21687521
DOI: 10.4161/adna.1.1.10952

実験手法

X-RAY DIFFRACTION (1.17 Å)