3FHP

A neutron crystallographic analysis of a porcine 2Zn insulin at 2.0 A resolution

3FHP の概要

• エントリーDOI: 10.2210/pdb3fhp/pdb
• 分子名称: Insulin, ZINC ION, ... (4 entities in total)
• 機能のキーワード: 2zn insulin, neutron crystallography, protonation, h/d exchange, carbohydrate metabolism, cleavage on pair of basic residues, glucose metabolism, hormone, secreted
• 由来する生物種: Sus scrofa (pig); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 11706.07

構造登録者

Iwai, W.,Kurihara, K.,Yamada, T.,Kobayashi, Y.,Ohnishi, Y.,Tanaka, I.,Takahashi, H.,Niimura, N. (登録日: 2008-12-09, 公開日: 2009-10-20, 最終更新日: 2024-11-20)

主引用文献

Iwai, W.,Yamada, T.,Kurihara, K.,Ohnishi, Y.,Kobayashi, Y.,Tanaka, I.,Takahashi, H.,Kuroki, R.,Tamada, T.,Niimura, N.
A neutron crystallographic analysis of T6 porcine insulin at 2.1 A resolution
Acta Crystallogr.,Sect.D, 65:1042-1050, 2009

PubMed Abstract: Neutron diffraction data for T(6) porcine insulin were collected to 2.1 A resolution from a single crystal partly deuterated by exchange of mother liquor. A maximum-likelihood structure refinement was undertaken using the neutron data and the structure was refined to a residual of 0.179. The hydrogen-bonding network of the central core of the hexamer was observed and the charge balance between positively charged Zn ions and their surrounding structure was interpreted by considering the protonation and/or deprotonation states and interactions of HisB10, water and GluB13. The observed double conformation of GluB13 was essential to interpreting the charge balance and could be compared with the structure of a dried crystal of T(6) human insulin at 100 K. Differences in the dynamic behaviour of the water molecules coordinating the upper and lower Zn ions were observed and interpreted. The hydrogen bonds in the insulin molecules, as well as those involving HisB10 and GluB13, are discussed. The hydrogen/deuterium (H/D) exchange ratios of the amide H atoms of T(6) porcine insulin in crystals were obtained and showed that regions highly protected from H/D exchange are concentrated in the centre of a helical region of the B chains. From the viewpoint of soaking time versus H/D-exchange ratios, the amide H atoms can be classified into three categories.

PubMed: 19770501
DOI: 10.1107/S090744490902770X

実験手法

NEUTRON DIFFRACTION (2 Å)