3FCA

Genetic Incorporation of a Metal-ion Chelating Amino Acid into proteins as biophysical probe

3FCA の概要

• エントリーDOI: 10.2210/pdb3fca/pdb
• 分子名称: Cysteine synthase, ZINC ION (3 entities in total)
• 機能のキーワード: phasing, heavy metal, unnatural amino acid, metal binding, transferase
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62605.53

構造登録者

Wang, F.,Lee, H.,Spraggon, G.,Schultz, P.G. (登録日: 2008-11-21, 公開日: 2009-02-17, 最終更新日: 2024-03-27)

主引用文献

Lee, H.S.,Spraggon, G.,Schultz, P.G.,Wang, F.
Genetic incorporation of a metal-ion chelating amino acid into proteins as a biophysical probe.
J.Am.Chem.Soc., 131:2481-2483, 2009

PubMed Abstract: A metal-ion chelating amino acid, (8-hydroxyquinolin-3-yl)alanine, was genetically encoded in E. coli by an amber nonsense codon and corresponding orthogonal tRNA/aminoacyl-tRNA synthetase pair. The amino acid was incorporated into TM0665 protein, and the mutant protein was cocrystallized with Zn(2+) to determine the structure by SAD phasing. The structure showed a high occupancy of the heavy metal bound to the HQ-Ala residue, and the heavy metal provided excellent phasing power to determine the structure. This method also facilitates the de novo design of metalloproteins with novel structures and functions, including fluorescent sensors.

PubMed: 19193005
DOI: 10.1021/ja808340b

実験手法

X-RAY DIFFRACTION (2.149 Å)